Article | . 2019 Vol. 37, Issue. 1
Evaluation of Cytotoxicity on Human Cancer Cells and Antioxidant and Immune Cell Activities of Codonopsis lanceolata Root Extracts Stored at Different Temperatures and for Different Durations



WELLPHYTO Co. Ltd., BI Center, GIST1
Seoul Center, Korea Basic Science Institute2
Faculty of Food Nutrition and Cookery, Woosong Information College3
Department of Industrial Plant Science & Technology, Chungbuk National University4
Department Horticulture Industry, College of Agriculture and Food Sciences, Wonkwang University5




2019.. 130:139


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Codonopsis lanceolata is widely consumed in South Korea; however, its medicinal applications have not been explored in detail. The present study aimed to evaluate the xanthine oxidase (XO) inhibitory activity, reducing power, immune activity, and cytotoxicity of 30% ethanolic extracts of C. lanceolata roots stored at different temperatures (5, 15, 25, 35, and 45°C) and for different storage periods (0, 15, 30, 45, 60, 75, and 90 days). The growth of human T and B cells increased when treated with the root extracts stored at low temperatures for a short period, in a concentration-dependent manner. The XO inhibitory activity of the C. lanceolata roots stored at 5°C and 15°C was higher than for those stored at 45°C. The XO inhibitory activity of the C. lanceolata roots gradually deceased with increasing storage period. The reducing power of the extracts increased significantly in a concentration-dependent manner. Reducing power of the root storage temperature for all temperatures and storage periods using 5 mg·mL-1 extract was higher than that for the other extract concentrations. Cytotoxic activity of C. lanceolata roots on human cancer cell lines (e.g., HeLa, Calu-6, and MCF-7) decreased as storage temperatures decreased from 45°C to 5°C. The cytotoxic effect on the MCF-7 cell line was relatively higher than that on the HeLa and Calu-6 cell lines. The results suggest that the root extract of C. lanceolata can be used to modulate immune response and is also a natural food additive with excellent physiological functionality.



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