Article | . 2018 Vol. 36, Issue. 6
Changes in the Activity of Peroxidase and Dehydrogenase in Pear Pollen During Germination under Different Storage Conditions

Department of Agricultural Chemistry, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agriculture and Life Sciences, Chonnam National University1
Department of Horticulture, Asian Pear Research Institute, College of Agriculture and Life Sciences, Chonnam National University2

2018.. 777:788


The duration of room temperature (RT) exposure directly affects the performance of pollen, and loss of pollen germination occurs within 12 h of exposure to RT. A germination rate of 55.9% was observed in the moisture-treated cold storage (MC) treatment. On the other hand, germination rate was 1.2% in the heat-treated frozen storage (HF) treatment. Furthermore, the germination rate of moisture pre-treatment groups including moisture-treated frozen storage (MF), MC, and moisture-treated RT storage (MR) was higher than that of the fresh frozen storage (FF) treatment (21.9%), especially those of the MC treatment, which exhibited up to a 2.5-fold increase. The activity of dehydrogenase in the MC treatment was 3-fold higher than that in the HF treatment. However, in the HF treatment, only marginal differences in the enzyme activity were observed among the tested conditions (FF, MF, MC, and MR). There was no linear relation between pollen germination and peroxidase (POD) activity (R2 = 0.3184). The activity of POD in pear pollen revealed no significant difference among the five storage conditions tested. In the HF treatment, POD activity was observed; however, the pollen grains were considered as non-viable. In conclusion, the duration of RT exposure negatively affects pollen germination, while the moisture pre-treatment impacts positively on pollen germination. The activity of POD is not required for, or associated with, the germination of pear pollen. Our results demonstrate that dehydrogenase is a potential indicator of pollen performance in pear. In this regard, viable pollen can be discriminated from non-viable pollen by monitoring their dehydrogenase activity.

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