Article | . 2018 Vol. 36, Issue. 1
Development of a Stem-color Mutant Kenaf (Hibiscus cannabinus L.) Cultivar, ‘Jeokbong’, and Analysis of its Functional Compounds

Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute1
Freshwater Bioresources Utilization Bureau, Nakdonggang National institute of Biological Resources2
Division of Plant Biotechnology, College of Agriculture and Life Science, Chonnam National University3

2018.. 77:81


A new cultivar, ‘Jeokbong’, was developed from kenaf cultivar ‘C14’ by gamma-ray (300 Gy) irradiation. ‘Jeokbong’ has several characteristics that distinguish it from its original cultivar, such as stem color (dark purple), leaf color (green purple), and its leaf shape. The flowering date was approximately 70 days after sowing, which was similar to that of the original cultivar. The dry matter yield of ‘Jeokbong’ was 14.8 ton·ha-1, which was also similar to that of the original cultivar. The anthocyanin composition of stem bark of ‘Jeokbong’ and three different kenaf cultivars (‘C14’, ‘Jangdae’, and ‘Hongma300’) were determined by HPLC-MS. A single pigment corresponding to delphinidin-3-O-sambubioside was identified in ‘Jeokbong’, but was not detected in ‘C14’, ‘Jangdae’, or ‘Hongma300’. The antioxidant and angiotensin converting enzyme inhibitory activity of ‘Jeokbong’ was approximately 4 times higher compared to the three other cultivars. The crude protein content of ‘Jeokbong’, ‘C14’, ‘Jangdae’, and ‘Hongma300’ were 9.2, 8.9, 10.5, and 10.6%, respectively. The crude fiber, neutral detergent fiber, and acid detergent fiber contents were not significantly different among the cultivars. These results suggest that ‘Jeokbong’ contains high levels of functional compounds and has unique ornamental value, and may be useful as a potential source of feed and as a landscape crop.

1. Abe N, Nemoto A, Tsuchiya Y, Hojo H, Hirota A (2000) Study of the 1.1-diphenyl-2-picrylhydrazyl radical scavenging mechanism for a 2-pyrone compound. Biosci Biotech Biochem 64:306-333. doi:10.1271/bbb.64.306  

2. Ali BH, Wabel N, Blunden G (2005) Phytochemical, pharmacological and toxicological aspects of Hibiscus sabdariffa L.: A review. Phytother Res 5:369-375. doi:10.1002/ptr.1628  

3. Alexopoulou E, Papattheohari Y, Christou M, Monti A (2013) Keanf: A multi-purpose crop for sever industrial applications. Springerverlag,London, England, pp 1-15. doi:10.1007/978-1-4471-5067-1_1  

4. AOAC (1990) Official methods of analyses, 15th ed. Association of Official Analytical Chemists, Washington DC, USA  

5. Chen C, Hu W, Zhang R, Jiang A, Zou Y (2016) Levels of phenolic compounds, antioxidant capacity, and microbial counts of fresh-cut onions after treatment with a combination of nisin and citric acid. Hortic Environ Biotechnol 57:266-273. doi:10.1007/s13580-016-0032-x  

6. Dempsey JM (1975) Fiber Crops. University Presses of Florida, Tallahassee, FL, USA, pp 203–304  

7. Jin CW, Ghimeray AK, Wang L, Xu ML, Piao JP, Cho DH (2013) Far infrared assisted kenaf leaf tea preparation and its effect on phenolic compounds, antioxidant and ACE inhibitory activity. J Med Plant Res 7:1121-1128  

8. Kang CH, Yoo YJ, Choi KH, Kim HJ, Shin YK, Lee GJ, Ko DY, Song YJ, Kim CK (2014) Analysis of ecotype, growth and development, yield and feed value of kenaf (Hibiscus cannabinus L.) genetic resources. Korean J Plant Res 27:556-566. doi:10.7732/kjpr.2014.27.5.556  

9. Kang SY, Kim DS, Lee GJ (2007) Genetic improvement of crop plants by mutation techniques in Korea. Plant Mutation Rep 1:7-15  

10. Kang SY, Kwon SJ, Jeong SW, Kim JB, Kim SH, Ryu J (2016) An Improved kenaf cultivar 'Jangdae' with seed harvesting in Korea. Korean J Breed Sci 48:349-354. doi:10.9787/KJBS.2016.48.3.349  

11. Kim BW, Sung KI, Nejad JG, Shin JS (2012) Nutritive value and fermentation quality of the silage of three kenaf (Hibiscus cannabinas L.) cultivars at three different growth stages. J Korean Soc Grassl Forage Sci 32:353-360. doi:10.5333/KGFS.2012.32.4.353  

12. Kim SH, Kim DS, Kim JB, Ha BK, Lee DM, Song HS, Kang SY (2015) Hibiscus syriacus ‘Dasom’, a new flower-color mutant variety developed by radiation breeding. Korean J Hortic Sci Technol 33:298-301. doi:10.7235/hort.2015.14160  

13. Koh GC (2011) Characteristics comparison of mutants induced through gamma Irradiation in ‘Kardinal’ rose. Korean J Hortic Sci Technol 29:456-460  

14. IAEA (2014) Mutant variety database (MVD). AboutMutantVarieties.aspx/  

15. Lee JY, Velusamy V, Koo JY, Ha HK, Kim DS, Kim JB, Kim SH, Kang SY (2012) Comparison of growth characteristics and chemical composition of kenaf (Hibiscus cannabinus L.) varieties as a potential forage crop. J Crop Sci 57:132-136. doi:10.7740/kjcs. 2012.57.2.132  

16. Mourtzinos I, Makris PD, Yannakopoulou K, Kalogeropouulos N, Michali I, Karathanos VT (2008) Thermal stability of anthocyanin extract of Hibiscus sabdariffa L. in the presence of β–cyclodextrin. Agric Food Chem 56:10303-10310. doi:10.1021/jf801389j  

17. Mumivand H, Babalar M, Tabrizi L, Craker LE, Shokrpour M, Hadian J (2017) Antioxidant properties and principal phenolic phytochemicals of Iranian tarragon (Artemisia dracunculus L.) accessions. Hortic Environ Biotechnol 58:414-422. doi:10.1007/s13580-017-0121-5  

18. Nakamura Y, Yamamoto N, Sakai K, Okubo A, Yamazaki S, Takano T (1995) Purification and characterization of angiotensin I-convertingenzyme inhibitors from sour milk. J Dairy Sci 78:777-783. doi:10.3168/jds.S0022-0302(95)76689-9  

19. Park KY, Hwang HJ, Chae WB, Choi GW (2014) Development of a new coreopsis variety ‘Uridream Pink’ by gamma-ray irradiation. Korean J Hortic Sci Technol 32:906-911. doi:10.7235/hort.2014.14074  

20. Ryu J, Ha BK, Kim DS, Kim JB, Kim SH, Kang SY (2013) Assessment of growth and seed oil composition of kenaf (Hibiscus cannabinus L.) germplasm. J Crop Sci Biotech 16:297-302. doi:10.1007/s12892-013-0074-x  

21. Ryu J, Kwon SJ, Ahn JW, Ha BK, Jeong SW, Im SB, Kim JB, Kim SH, Lee YK, et al (2016) Evaluation of nutritive value and identification of fungi in silage from new kenaf (Hibiscus cannabinus L.) cultivars. Int J Agric Biol 18:1159-1168. doi:10.17957/IJAB/15.0220  

22. Song TH, Han OK, Park TL, Kim YK, Kim KJ, Park KH (2012) Effect of nitrogen top dressing levels on productivity, feed value, and anthocyanin content of colored Barley. J Korean Soc Grassl Forage Sci 32:149-156. doi:10.5333/KGFS.2012.32.2.149