Article | . 2017 Vol. 35, Issue. 5
Effect of After-Ripening, Cold Stratification, and GA3 Treatment on Lychnis wilfordii (Regel) Maxim Seed Germination



Useful Plant Resources Center, Korea National Arboretum1
Department of Horticulture and Forestry, Pai Chai University2
Garden and Education Division, Korea National Arboretum3




2017.. 525:533


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Lychnis wilfordii (Regel) Maxim is an endemic plant species that occurs in the wetlands of Gangwon-do, Korea. This research was conducted to investigate the effect of after-ripening treatment (0, 3, 6, or 9 weeks at 20-25°C), cold stratification (0, 3, 6, or 9 weeks at 5°C), and GA3 treatment (0, 10, 100, or 1000 mg·L-1) on L. wilfordii seed germination. Seeds were transferred to germination conditions (20°C, 12 h photoperiod of florescent lamps with 26.1 μmol·m-2·s-1 PAR or darkness) following after-ripening, cold stratification, or GA3 treatment. In after-ripening experiments, the final germination percentage (FGP) of seeds with 0, 3, 6, and 9 weeks of after-ripening was 0, 0.7, 2.3, and 33.2% in the light condition and 1.6, 1.9, 2.3, and 22.6% in darkness, respectively. In cold stratification experiments, the FGP of seeds treated with 0, 3, 6, and 9 weeks of clod stratification was 0, 80.5, 77.0, and 83.3% in light conditions and 1.6, 77.8, 58.4, and 83.1% in darkness conditions, respectively. The mean germination time of 1,000 mg·L-1 GA3 treatment was approximately five days in light and three days in darkness conditions, respectively. These results indicate that seed germination in L. wilfordii can be improved with 1,000 mg·L-1 GA3 and cold stratification (3 or 6 weeks) was effective for the mass propagation of L. wilfordii.



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