Article | . 2017 Vol. 35, Issue. 4
Morphological Variation of Two Cultivated Types of Perilla Crop from Different Areas of China

Department of Applied Plant Sciences, College of Agriculture and Life Sciences, Kangwon National University1

2017.. 510:522


To better understand the morphological variation for Perilla crop in different areas of China, we studied the morphological variation in 87 accessions (84 cultivated var. frutescens and three cultivated var. crispa ) from high latitude (Northeast China) and middle latitude (North and Northwest China) areas of China by examining seven quantitative and 10 qualitative characters. Analysis of the morphological variation determined that there was significant morphological differences in five quantitative traits between cultivated var. frutescens and cultivated var. crispa , including effective number of branches (QN2), number of internodes (QN3), number of branches (QN4), length of the largest inflorescence (QN5), and days from germination to flowering (QN7). However, two quantitative traits-plant height and number of florets of the largest inflorescence-did not show any significant differences between cultivated var. frutescens and cultivated var. crispa . In addition, significant differences for six quantitative traits were found between the accessions of cultivated var. frutescens originating from high and middle latitude areas in China, which included QN2, QN3, QN5, number of florets of the largest inflorescence (QN6), and QN7. Principal components analysis (PCA) identified five quantitative characters [plant height (QN1), QN2, QN3, QN4, QN7] and six qualitative characters [fragrance of plant (QL1), color of reverse side of leaf (QL3), degree of pubescence (QL5), color of flower (QL6), shape of leaf (QL7), and hardness of seed (QL10)] that contributed to the positive direction on the first axis. The other quantitative and qualitative characters contributed to the negative direction on the first axis. Most accessions of cultivated var. frutescens and cultivated var. crispa were clearly separated by the first axis. In addition, most accessions of cultivated var. frutescens are from high latitude and middle latitude areas that were clearly separated by the first axis, except for several accessions. The findings from this study will provide useful information towards understanding the morphological variation of Perilla crop according to geographical distribution in high and middle latitude regions of China.

1. Aston DL, Bradshaw AD (1966) Evolution in closely adjacent populations. II Agrostis stolonifera in maritime habitats. Heredity 21:649–664. doi:10.1038/hdy.1966.64  

2. Al-Hiyaly SEK, McNeilly T, Bradshaw AD (1993) The effect of zinc contamination from electricity pylons. Genetic constraints on selection for zinc tolerance. Heredity 70:22-32. doi:10.1038/hdy.1993.4  

3. Brush SB (1995) In situ conservation of landraces in centers of crop diversity. Crop Sci 35:346-354. doi:10.2135/cropsci1995.0011183X003500020009x  

4. Chen T, Li L, Zhang J, Huang ZL, Zhang HW, Liu Y, Chen Q, Tang HR, Wang XR (2016) Investigation, collection and preliminary evaluation of genetic resources of Chinese cherry [Cerasus pseudocerasus (Lindl.) G. Don]. J Fruit Sci 33:917-933  

5. Duminil J, Hardy OJ, Petit RJ (2009) Plant traits correlated with generation time directly affect inbreeding depression and mating system and indirectly genetic structure. BMC Evol Biol 9:177. doi:10.1186/1471-2148-9-177  

6. Gould SJ, Johnston RF (1972) Geographic variation. Ann Rev Ecol Syst 3:457-498  

7. Harlan JR (1992) Origins and processes of domestication. In GP Chapman, ed, Grass Evolution and Domestication, Cambridge University Press, Cambridge, UK, pp 159-175  

8. IPGRI (1994) In situ conservation of crop and agroforestry species. Prepared for the CGIAR Mid-Term Meeting, 23-26 May 1994, New Delhi, India  

9. Lee JK, Nitta M, Kim NS, Park CH, Yoon KM, Shin YB, Ohnishi O (2002) Genetic diversity of Perilla and related weedy types in Korea determined by AFLP analyses. Crop Sci 42:2161-2166. doi:10.2135/cropsci2002.2161  

10. Lee JK, Ohnishi O (2001) Geographical differentiation of morphological characters among and their weedy types in East Asia. Breed Sci 51:247-255. doi:10.1270/jsbbs.51.247  

11. Lee JK, Ohnishi O (2003) Genetic relationships among cultivated types of Perilla frutescens and their weedy types in East Asia revealed by AFLP markers. Genet Resour Crop Evol 50:65-74. doi:10.1023/A:1022951002271  

12. Luo L, Wang JN, Kong LD, Jiang QG, Tan RX (2000) Antidepressant effects of Banxia Houpu decoction, a traditional Chinese medicinal empirical formula. J Ethnopharmacol 73:277-281. doi:10.1016/S0378-8741(00)00242-7  

13. Makino T, Hara H, Tuyama T, Fumio M (1961) Makino’s New Illustrated Flora of Japan. Hokuryukan, Tokyo, Japan, p 1060  

14. Mao QQ, Huang Z, Zhong XM, Feng CR, Pan AJ, Li ZY, Ip SP, Che CT (2010) Effects of SYJN, a Chinese herbal formula, on chronic unpredictable stress induced changes in behavior and brain BDNF in rats. J Ethnopharmacol 128:336-341. doi:10.1016/j.jep.2010.01.050  

15. Meng L, Lozano Y, Bombarda I, Gaydou EM, Li B (2009) Polyphenol extraction from eight Perilla frutescens cultivars. C R Chim 12:602-611. doi:10.1016/j.crci.2008.04.011  

16. Meng F, Liu L, Peng M, Wang Z, Wang C, Zhao Y (2015) Genetic diversity and population structure analysis in wild strawberry (Fragaria nubicola L.) from Motuo in Tibet Plateau based on simple sequence repeats (SSRs). Biochem Syst Ecol 63:113-118. doi:10.1016/j.bse.2015.09.018  

17. Nitta M (2001) Origin and their weedy type. PhD Thesis, Kyoto University, Kyoto, Japan, p 78  

18. Nitta M, Lee JK, Kobayashi H, Liu D, Nagamine T (2005) Diversification of multipurpose plant, Perilla frutescens. Genet Resour Crop Evol 52:663-670. doi:10.1007/s10722-003-6013-9  

19. Nitta M, Lee JK, Ohnishi O (2003) Asian Perilla crops and their weedy forms: Their cultivation, utilization and genetic relationships. Econ Bot 57:245-253. doi:10.1663/0013-0001(2003)057[0245:APCATW]2.0.CO;2  

20. Nitta M, Ohnishi O (1999) Genetic relationships among two , shiso and egoma, and the weedy type revealed by RAPD markers. Jpn J Genet 74:43-48. doi:10.1266/ggs.74.43  

21. Rao VR, Hodgkin T (2002) Genetic diversity and conservation and utilization of plant genetic resources. Plant Cell Tissue Organ Cult 68:1-19  

22. Sa KJ, Choi SH, Ueno M, Lee JK (2015) Genetic diversity and population structure in cultivated and weedy types of Perilla in East Asia and other countries as revealed by SSR markers. Hortic Environ Biotechnol 56:524-534. doi:10.1007/s13580-015-0039-8  

23. Schwanitz F (1966) The Origin of Cultivated Plants. Harvard University Press, Cambridge, MA USA, p 175  

24. Tan M, Yan M, Wang L, Wang L, Yan X (2012) Research progress on Perilla frutescens . Chinese J Oil Crop Sci 34:225-231  

25. Wyatt R, Antonovics J (1981) Butterfly weed re-revisited: Spatial and temporal patterns of leaf shape variation in Asclepias tuberosa . Evolution 35:529-542. doi:10.1111/j.1558-5646.1981.tb04915.x  

26. Wu S, Wu M, Quan X, Cai C (2005) High-yield cultivation technique of Perilla frutescens (Linn.) Britt. in Yanbian area. J Agric Sci Yanbian Univ 27:48-55  

27. Yu H, Qiu JF, Ma LJ, Hu YJ, Li P, Wang JB (2016) Phytochemical and phytopharmacological review of Perilla frutescens L. (Labiatae), a traditional edible-medicinal herb in China. Food Chem Toxicol doi:10.1016/j.fct.2016.11.023  

28. Zhang J, Chen T, Wang J, Chen Q, Luo Y, Zhang Y, Tang H (2016) Genetic diversity and population structure in cherry [Cerasus pseudocerasus (Lindl). G. Don] along Longmenshan Fault Zones in China with newly developed SSR markers. Sci Hortic 212:11- 19. doi:10.1016/j.scienta.2016.09.033  

29. Zohary D, Hopf M (1993) Domestication of plants in the old world. Clarendon Press, Oxford, UK, p 249