Article | . 2017 Vol. 35, Issue. 4
Effects of Light-emitting Diodes on In Vitro Growth of Virus-free Sweet Potato Plantlets



Institute of Life Science and Natural Resources, Wonkwang University1




2017.. 465:479


PDF XML




The in vitro growth of virus-free sweet potato [Ipomoea batatas (L.) Lam.] plantlets was investigated under different light sources: fluorescent lamp (control); red (660 nm), blue (460 nm), white light-emitting diodes (LED), and two mixtures of blue and red LED (R:B = 8:2, and 7:3). Single node explants (10 mm) of three cultivars (‘Matnami’, ‘Shincheonmi’, and ‘Yeonhwangmi’) were cultured on Murashige and Skoog medium supplemented with 0.2mg·L-1 6-benzyladenine for 4 weeks. Explants were exposed to 150 ± 5 μmol·m-2·s-1photosynthetic photon flux at a distance of 20 cm, constant temperature of 25°C, and under 16/8-h (day/ night) photoperiod. Using the same method, the in vitro growth of 10 cultivars under red LED was also compared. After 3 weeks, vine length was highest in plantlets cultured under red LED, and lowest in plantlets cultured under blue LED. Fresh and dry weights were also greatest in plantlets cultured under red LED. Compared to the control, vine thickness was significantly higher in plantlets grown under white LED and the 7:3 R:B LED mixture. Significant differences were observed among the 10 cultivars grown under red LED. ‘Matnami’, ‘Shincheonmi’, and ‘Shinhwangmi’ all had excellent vine lengths, and fresh and dry weights. Compared to the control, vine elongation of sweet potato plantlets was most effective under red LED, and culture duration was about 1 week shorter.



1. Bourget CM (2008) An introduction to light-emitting diodes. HortScience 43:1944-1946  

2. Briggs WR (1993) New light on stem growth. Nature 366:110-111. doi:10.1038/366110a0  

3. Budiarto K (2010) Spectral quality affects morphogenesis on anthurium plantlet during in vitro culture. AGRIVITA 32:234-240  

4. Cha MK, Cho JH, Cho YY (2013) Growth of leaf lettuce as affected by light quality of LED in closed-type plant factory system. Protected Hortic Plant Fac 22:291-297. doi:10.12791/KSBEC.2013.22.4.291  

5. Cheong EJ, Hurtt S, Salih S, Li R (2010) Development of a reliable technique to eliminate sweet potato leaf curl virus through meristem tip culture combined with therapy of infected Ipomoea species. Korean J Plant Res 23:233-241  

6. Chung MN (2008) A study on the virus detection methods and virus-free plant mass production in sweetpotato. PhD Thesis, Chonnam National University, Gwangju, Korea  

7. Choi MK, Baek GY, Kwon SJ, Yoon YC, Kim HT (2014) Effect of LED light wavelength on lettuce growth, vitamin C and anthocyanin contents. Protected Hortic Plant Fac 23:19-25. doi:10.12791/KSBEC.2014.23.1.019  

8. Choi YW, Ahn CK, Kang JS, Son BG, Choi IS (2003) Growth, photomorphogenesis, and photosynthesis of Perilla grown under red, blue light emitting diodes and light intensities. J Kor Soc Hortic Sci 44:281-286  

9. Hahn EJ, Kozai T, Paek KY (2000) Blue and red light-emitting diodes with or without sucrose and ventilation affect in vitro growth of Rehmannia glutinosa plantlets. J Plant Biol 43:247-250.doi:10.1007/BF03030425  

10. Heo JW, Lee CW, Chakrabarty D, Paek YK (2002) Growth responses of marigold and salvia bedding plants as affected by monochromic or mixture radiation provided by a light emitting diode (LED). Plant Growth Regul 38:225–230. doi:10.1023/A:1021523832488  

11. Im JU, Yoon YC, Soe KW, Kim KH, Moon AK, Kim HT (2013) Effect of LED light wavelength on chrysanthemum growth. Protected Hortic Plant Fac 22:49-54. doi:10.12791/KSBEC.2013.22.1.049  

12. Karyeija RF, Gibson RW, Valkonen JPT (1998) The significance of sweet potato feathery mottle virus in subsistence sweet potato production in Africa. Plant Dis 82: 4-15. doi:10.1094/PDIS.1998.82.1.4  

13. Kim EY, Park SA, Park BJ, Lee Y, Oh MM (2014) Growth and antioxidant phenolic compounds in cherry tomato seedlings grown under monochromatic light-emitting diodes. Hortic Environ Biotechnol 55:506-513. doi:10.1007/s13580-014-0121-7  

14. Kim HH, Goins GD, Wheeler RM, Sager JC (2004a) Green-light supplementation for enhanced lettuce growth under red- and bluelight- emitting diodes. HortScience 39:1617-1622  

15. Kim HR, You YH (2013) Effects of red, blue, white, and far-red LED source on growth responses of Wasabia japonica seedlings in plant factory. Korean J Hortic Sci Technol 31:415-422. doi:10.7235/hort.2013.13011  

16. Kim SJ, Hahn EJ, Heo JW, Paek KY (2004b) Effects of LED on net photosynthetic rate, growth and leaf stomata of chrysanthemum plantlets in vitro. Sci Hortic 101:143-151. doi:10.1016/j.scienta.2003.10.003  

17. Kurilcik A, Miklusyte-Canova R, Dapkuniene S, Zilinskaite S, Kurilcik G, Tamulaitis G, Duchovskis P, Zukauskas A (2008) In vitro culture of chrysanthemum plantlets using light-emitting diodes. Cent Eur J Biol 3:161-167. doi:10.2478/s11535-008-0006-9  

18. Lee JG, Oh SS, Cha SH, Jang YA, Kim SY, Um YC, Cheong SR (2010) Effects of red/blue light ratio and short-term light quality conversion on growth and anthocyanin contents of baby leaf lettuce. J Bio-Environ Control 19:351-359  

19. Lee JS, Kim YH (2014) Growth and anthocyanins of lettuce grown under red or blue light-emitting diodes with distinct peak wavelength. Korean J Hortic Sci Technol 32:330-339. doi:10.7235/hort.2014.13152  

20. Lee JS, Lee HI, Kim YH (2012) Seedling quality and early yield after transplanting of paprika nursed under light-emitting diodes, fluorescent lamps and natural light. J Bio-Environ Control 21:220-227  

21. Lee NR, Lee SY (2014) Growth and tuber yield of sweet potato slips grown under different light-emitting diodes. Protected Hortic Plant Fac 23:356-363. doi:10.12791/KSBEC.2014.23.4.356  

22. Li H, Xu Z, Tang C (2010) Effect of light-emitting diodes on growth and morphogenesis of upland cotton (Gossypium hirsutum L.) plantlets in vitro. Plant Cell Tiss Organ Cult 103:155-163. doi:10.1007/s11240-010-9763-z  

23. Lian ML, Murthy HN, Paek KY (2002) Effect of light emitting diodes (LED) on the in vitro induction and growth of bulblets of Lilium oriental hybrid ‘Pesaro’. Sci Hortic 94:365-370. doi:10.1016/S0304-4238(01)00385-5  

24. Manivannan A, Soundararajan P, Halimah N, Chung HK, Jeong BR (2015) Blue LED light enhances growth, phytochemical contents, and antioxidant enzyme activities of Rehmannia glutinosa cultured in vitro. Hortic Environ Biotechnol 56:105-113. doi:10.1007/s13580-015-0114-1  

25. Manivannan A, Soundararajan P, Park YG, Wei H, Kim SH, Jeong BR (2017) Blue and red light-emitting diodes improve the growth and physiology of in vitro-grown carnations ‘Green Beauty’ and ‘Purple Beauty’. Hortic Environ Biotechnol 58, 12-20. doi:10.1007/s13580-017-0051-2  

26. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473- 497. doi:10.1111/j.1399-3054.1962.tb08052.x  

27. Nam SS, Chung MN, Lee JS, Lee HU, Yang JW, Hwang EJ, Lee KB (2016) Effect of liquid culture on the propagation of virus free plantlets from meristem culture-derived of sweet potato [Ipomoea batatas L. (Lam)]. Korean Soc Int Agric 28:215-219. doi:10.12719/KSIA.2016.28.2.215  

28. Nhut DT, Takamura T, Watanabe H, Tanaka M (2003a) Efficiency of a novel culture system by using light-emitting diode (LED) on in vitro and subsequent growth of micropropagated banana plantlets. Acta Hortic 616:121-127. doi:10.17660/ ActaHortic.2003.616.10  

29. Nhut DT, Takamura T, Watanabe H, Okamoto K, Tanaka M (2003b) Responses of strawberry plantlets cultured in vitro under superbright red and blue light-emitting diodes (LED). Plant Cell Tiss Org Cult 73:43–52. doi:10.1023/A:1022638508007  

30. Nhut DT, Takamura T, Watanabe H, Okamoto K, Tanaka M (2005) Artificial light source using light-emitting diodes (LED) in the efficient micropropagation of Spathiphyllum plantlets. Acta Hortic 692:137-142. doi:10.17660/ActaHortic.2005.692.18  

31. Poudel PR, Kataoka I, Mochioka R (2008) Effect of red- and blue-light-emitting diodes on growth and morphogenesis of grapes. Plant Cell Tiss Org Cult 92:147–153. doi:10.1007/s11240-007-9317-1  

32. Ryu JH, Seo KS, Choi GL, Rha ES, Lee SC, Choi SK, Kang SY, Bae CH (2012) Effects of LED light illumination on germination, growth and anthocyanin content of dandelion (Taraxacum officinale ). Korean J Plant Res 25:731-738. doi:10.7732/kjpr.2012.25.6.731  

33. Saebo A, Krekling T, Appelgren M (1995) Light quality affects photosynthesis and leaf anatomy of birch plantlets in vitro. Plant Cell Tiss Org Cult 41:177–185. doi:10.1007/BF00051588  

34. Shin HM (2011) Propagation of virus-free sweet potato plant. Chungcheongbuk-do Agricultural Research and Extension Services Research Report, 2011. p 42-47  

35. Shin KS, Murthy HN, Heo JW, Hahn EJ, Paek KY (2008) The effect of light quality on the growth and development of in vitro cultured Doritaenopsis plants. Acta Physiol Plant 30:339-343. doi:10.1007/s11738-007-0128-0  

36. Shin YS, Lee MJ, Lee ES, Ahn JH, Lim JH, Kim HJ, Park HW, Um YG, Park SD, et al. (2012) Effect of LED (light emitting diodes) irradiation on growth and mineral absorption of lettuce (Lactuca sativa L. ‘Lollo Rosa’). J Bio-Environ Control 21:180-185  

37. Son KH, Park JH, Kim DI, Oh MM (2012) Leaf shape, growth, and phytochemicals in two leaf lettuce cultivars grown under monochromatic light-emitting diodes. Korean J Hortic Sci Technol 30:664-672. doi:10.7235/hort.2012.12063  

38. Tennessen DJ, Singsaasl EL, Sharkeyl TD (1994) Light-emitting diodes as a light source for photosynthesis research. Photosyn Res 39:85-92. doi:10.1007/BF00027146  

39. Yoo KR, Lee SY (2013) Growth characteristics and yield of sweet potato cultivars between virus-free and farmer’ slips in late season cultivation. Korean J Crop Sci 58:43-49. doi:10.7740/kjcs.2013.58.1.043  

40. Yorio NC, Goins GD, Kagie HR, Wheeler RM, Sager JC (2001) Improving spinach, radish, and lettuce growth under red light-emitting diodes (LED) with blue light supplementation. HortScience 36:380-383