Article | . 2017 Vol. 35, Issue. 4
Culture Conditions Affecting Spore Germination, Prothallus Propagation and Sporophyte Formation of Dryopteris nipponensis Koidz.



Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University1
Major in Horticulture, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University2
Useful Plant Resources, Korea National Arboretum of the Korea Forest Service3




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We investigated a suitable method for in vitro germination of spores, propagation of prothalli, and the formation of sporophytes in the fern Dryopteris nipponensis Koidz. Spore germination rate was relatively high regardless of culture medium. Prothallus development was faster in Knop medium than in Murashige and Skoog (MS) media. Prothalli used in all experiments were obtained from germinated spores, and were cultivated in different concentrations of media components. The active formation of sexual organs such as antheridium made 1MS medium suitable for prothallus propagation, although there was a lower propagation ratio compared to Knop medium. Growth and morphogenesis of prothalli were most effective on 1MS medium containing 2% sucrose, and 60 mM of total nitrogen source with 20:40 mM ratio of NH4 +:NO3 - To select a suitable soil composition for sporophyte formation, ground prothalli were cultivated on single and mixed soils using bed soil, peat moss, perlite, and decomposed granite for 14 weeks. Bed soil promoted sporophyte formation and growth regardless of single or mixed use. In particular, a mixture of bed soil and decomposed granite in a 2:1 ratio (v:v) led to accelerated sporophyte formation (0.83/cm2).



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