Article | . 2017 Vol. 35, Issue. 1
Effects of Medium Components and Composition on Mass Propagation of Arachniodes aristata (G. Forst.) Tindale



Department of Horticultural Science, Chungbuk National Universtiy,1
Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University2




2017.. 131:141


PDF XML




This study was conducted to investigate in vitro mass propagation methods suitable for each growth stage of A. aristata (G. Forst.) Tindale, from spore germination to sporophyte formation. Among spores germinated in 1/8 - 1 × MS medium and Knop medium, Knop medium yielded the highest germination percentage (87.1%). We cultured prothalli obtained from germinating spores for 8 weeks on media with different concentrations of sucrose and active carbon, as well as different concentrations and ratios of nitrogen, to select a suitable growth medium. A. aristata (G. Forst.) Tindale prothalli grew most actively in MS medium with 3% sucrose and 20 : 40 mM of NH4Cl and KNO3 (total concentration of 60 mM). We investigated sporophyte formation according to soil type, finding that bedding soil mixed with perlite at a 2 : 1(v / v) ratio yielded the highest number of sporophytes per pot (73.8 / 7.5 × 7.5 cm pot). By contrast, when peat moss was used alone or mixed with other substrates, prothallus development and sporophyte formation were suppressed. Therefore, the most effective propagation method for A. aristata (G. Forst.) Tindale is to grow prothalli in MS medium and to induce sporophyte formation in a mixture of bedding soil and perlite (v / v = 2 : 1).



1. Bang KJ, Ju JH (2002) Effects of indoor relative humidity conditions on the growth of and in native ferns. J Korean Env Res & Reveg Technol 5:34-38  

2. Bertrand AM, Albuerne MA, Fernandez H, Gonzalez A, Sanchez-Tames R (1999). organogenesis of .  

3. Cox J, Bhatia P, Ashwath N (2003) spore germination of the fern . Sci Hort 97:369-378. doi:10.1016/S0304-4238(02)00152-8  

4. Czaja AT (1921). Uber befruchtung, bastardierung and geschlechtertrennung bei prothallien homosporer Farne. Z Bot 13:545-589  

5. Dyer AF (1979) The culture of fern gametophytes for experimental investigation. Dyer AF ed, The Experimental Biology of Ferns.Academic press, London, pp. 253-305  

6. Fernandez H, Bertrand AM, Sanchez-Tames R (1999) Biological and nutritional aspects in fern multiplication. Plant Cell Tiss Org Cult 56:211-214. doi: 10.1023/A:1006277229136  

7. Higuchi H, Amaki W (1989) Effect of 6-benzylaminopurine on the organogenesis of L. through propagation.Sci Hort 37:351-359. doi: 10.1016/0304-4238(89)90146-5  

8. Ju JH, Bang KJ (2005) Effects of irrigation interval, medium composition and drainage on the growth response of Korea native fern at indoor. J Kor Inst Landscape Archit 32:103-108  

9. Korea Fern Society (KFS) (2005). Illustrated fern native to Korea. Geobook, Seoul   

10. Laurent S, Lefebvre MF (1980) Etude de leffet de differentes carences en elemets mineraux sur la teneur en tanoides de gametophytes de filicinees. Bull Soc Bot Fr Lett Bot 127:119-127  

11. Lee CH (2004) Propagation and technique of masspropagation of Pteridophyta native to Korea. Kor Wild Res Assn 3:91-96 Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473- 497. doi: 10.1111/j.1399-3054.1962.tb08052.x  

12. Padhya MA, Mehta AR (1982) Propagation of fern () through tissue culture. Plant Cell Rep 1:261-263. doi:10.1007/BF00272634  

13. Perez-Garcia B, Mendoza-Ruiz A, Espinosa-Matias S, Gomez-Pianataro LD (2010) Gametophyte morphology of Baker and Platycerium wandae Racif. Micron 41:806-813. doi: 10.1016/j.micron.2010.05.005  

14. Schwabe WW (1951) Physiological studies in plant nutrition XVI. The mineral nutrition of bracken. Ann Bot 15:417-446   

15. Shin HC, Park NC, Choi KO (2010) Light responses and introduction plan of the native evergreen 'Filicales plant' into the interior landscape space. J Kor Soc People Plants Env 13:109-116  

16. Shin SL (2007) Several factors affecting masspropagation of eight fern species. MS Thesis, Chungbuk Natl Univ Cheongju,Korea  

17. Takahashi N, Hashino M, Kami C, Imaichi R (2009) Developmental morphology of strap-shaped gametophytes of : a new look at meristem development and function in fern gametophytes. Ann Bot 104:1353-1561. doi: 10.1093/aob/mcp245   

18. Toole EH and Tottingham WE (1918) The influence of certain added solids upon the composition and efficiency of Knop's nutrient solution. Am J Bot 5:452-461. doi: 10.2307/2435152  

19. Whittier DP (1975) The influence of osmotic conditions on induced apogamy in gametophytes. Phytomorphology 25:246-249