Article | . 2017 Vol. 35, Issue. 1
Inoculation with Bacillus licheniformis MH48 Promotes Nutrient Uptake in Seedlings of the Ornamental Plant Camellia japonica grown in Korean Reclaimed Coastal Lands

Division of Forest Resources, College of Agriculture and Life Sciences, Chonnam National University1
Division of Food Technology, Biotechnology and Agrochemistry, Institute of Environmentally-Friendly Agriculture, Chonnam National University2
Purne Co., Ltd., Institute of Environmentally-Friendly Agriculture, Chonnam National University3
Division of Forest Restoration, National Institute of Forest Science4
Division of Plant Biotechnology, College of Agriculture and Life Sciences, Chonnam National University5
Department of Forest Environmental Science, College of Agriculture and Life Science, Chonbuk National University6

2017.. 11:20


The objective of this study was to determine whether inoculation with Bacillus licheniformis MH48 as a plant growth-promoting rhizobacterium (PGPR) could promote nutrient uptake of seedlings of the ornamental plant Camellia japonica in the Saemangeum reclaimed coastal land in Korea. B. licheniformis MH48 inoculation increased total nitrogen and phosphorus content in soils by 2.2 and 20.0 fold, respectively, compared to those without bacterial inoculation. In addition, B. licheniformis MH48 produced auxin, which promoted the formation of lateral roots and root hairs, decreased production of growth-inhibiting ethylene, and alleviated salt stress. Total nitrogen and phosphorus uptake of seedlings subjected to bacterial inoculation was 2.3 and 3.6 fold higher, respectively, than the control. However, B. licheniformis MH48 inoculation had no significant effect on the growth of seedlings. Our results suggest that inoculation with B. licheniformis MH48 can be used as a PGPR bio - enhancer to stimulate fine root development, promote nutrient uptake and alleviate salt stress in ornamental plant seedlings grown in the high-salinity conditions of reclaimed coastal land.

1. Abbasi MK, Sharif S, Kazmi M, Sultan T, Aslam M (2011) Isolation of plant growth promoting rhizobacteria from wheat rhizosphere and their effect on improving growth, yield, and nutrient uptake of plants. Plant Biosyst 145:159-168. doi:10.1080/11263504.20 10.542318  

2. Adesemoye AO, Kloepper JW (2009) Plant-microbes interactions in enhanced fertilizer use efficiency. Appl Microbiol Biotechnol 85:1-12. doi:10.1007/s00253-009-2196-0  

3. Aslantas R, Cakmakci R, Sahin F (2007) Effect of plant growth promoting rhizobacteria on young apple tree growth and fruit yield under orchard conditions. Sci Hortic 111:371-377. doi:10.1016/j.scienta.2006.12.016  

4. Beneduzi A, Peres D, Vargas LK, Bodanese-Zanettini MH, Passaglia LMP (2008) Evaluation of genetic diversity and plant growth promoting activities of nitrogen-fixing Bacilli isolated from rice fields in South Brazil. Appl Soil Ecol 39:311-320. doi:10.1016/ j.apsoil.2008.01.006  

5. Cho DO (2007) The evolution and resolution of conflicts on Saemangeum Reclamation Project. Ocean Coast Manage 50:930-944.doi: 10.1016/j.ocecoaman.2007.02.005  

6. Dö bereiner J (1997) Biological nitrogen fixation in the tropics: social and economic contributions. Soil Biol Biochem 2:771-774. doi:10.1016/S0038-0717(96)00226-X  

7. Esitken A, Pirlak P, Turan M, Sahin F (2006) Effects of floral and foliar application of plant growth promoting rhizobacteria (PGPR) on yield, growth of nutrition of sweet cherry. Sci Hortic 1:324-327. doi:10.1016/j.scienta.2006.07.023   

8. Frankenberger WT, Brunner W (1982) Method of detection of auxin-indole-3-aceticacid in soil by high performance liquid chromatography. Soil Sci Soc Am J 47:237-241. doi:10.2136/sssaj1983.03615995004700020012x  

9. Glick BR, Penrose DM, Li J (1998) A model for the lowering of plant ethylene concentrations by plant growth promoting bacteria. JTheor Biol 190:63-68. doi:10.1006/jtbi.1997.0532  

10. Kohler J, Hernandez JA, Caravaca F, Roldan A (2009) Induction of antioxidant enzymes is involved in the greater effectiveness of a PGPR versus AM fungi with respect to increasing the tolerance of lettuce to severe salt stress. Environ Exp Bot 65:245-252. doi:10.1016/j.envexpbot.2008.09.008  

11. Korea Meteorological Administration (2016) The past climate data in Korea. Accessed 2 February 2016. (in Korean)   

12. Korea Soil Information System (2016) Soil environment map. Accessed 2 February 2016. (in Korean)   

13. Koyro HW (2006) Effect of salinity on growth, photosynthesis, water relations and solute composition of the potential cash crop halophyte (L.). Environ Exp Bot 56:136-146. doi:10.1016/j.envexpbot.2005.02.001   

14. Lee RDW, Cho HT (2013) Auxin, the organizer of the hormonal/environmental signals for hair growth. Front Plant Sci 4:448. doi:10.3389/fpls.2013.00448  

15. Leitner D, Klepsch S, Ptashnyk M, Marchant A, Kirk GJD, Schnepf A, Roose T (2010) A dynamic model of nutrient uptake by root hairs.New Phytol 185:792-802. doi:10.1111/j.1469-8137.2009.03128.x   

16. Lie HJ, Cho CH, Lee S, Kim ES, Koo BJ, Noh JH (2008) Changes in marine environment by a large coastal development of the Saemangeum reclamation project in Korea. Ocean Polar Res 30:475-484. doi:10.4217/OPR.2008.30.4.475   

17. Liu F, Xing S, Ma H, Du Z, Ma B (2013) Plant growth-promoting rhizobacteria affect the growth and nutrient uptake of Fraxinus americana container seedlings. Appl Microbiol Biotechnol 97:4617-4625. doi:10.1007/s00253-012-4255-1   

18. Mohamed SSE, Babiker HM (2012) Effects of Rhizobium inoculation and urea fertilization on faba bean ( L.) production in a semi-desert zone. Adv Environ Biol 6:824-830  

19. Mohamed HI, Gomaa EZ (2012) Effects of plant growth promoting Bacillus subtilis and Pseudomonas fluorescens on growth and pigment composition of radish plants ( ) under NaCl stress. Photosynthetica 50:263-272. doi:10.1007/s11099-012-0032-8  

20. Mulvaney RL (1996) Nitrogen inorganic forms. In DL Spark, AL Page, PA Helmke, RH Loeppert, PN Soltanpoor, MA Tabatabai, CT   

21. Johnston, ME Sumner, eds, Methods of soil analysis: Part 3 Chemical methods. Soil Science Society of America, Wisconsin, USA,pp 1123-1184  

22. Orhan E, Esitken A, Ercisli S, Turan M, Sahin F (2006) Effects of plant growth promoting rhizobacteria (PGPR) on yield, growth and nutrient contents in organically growing raspberry. Sci Hortic 111:38-43. doi:10.1016/j.scienta.2006.09.002   

23. Parida AK, Das AB (2005) Salt tolerance and salinity effects on plants: a review. Ecotox Environ Safe 60:324-349. doi:10.1016/j.ecoenv.2004.06.010  

24. Park H (2016) Plant growth promoting rhizobacteria affect soil fertility and growth environment of seedlings in Saemangeum coastal reclaimed land of Korea. Master’s thesis, Chonnam National University, pp 1-27  

25. Patten CL, Glick BR (2002) Role of putidaindoleacetic acid in development of the host plant root system. Appl Environ Microb 68:3795-3801. doi:10.1128/AEM.68.8.3795-3801.2002  

26. Prunty L, Greenland R (1997) Nitrate leaching using two potato-corn N-fertilizer plans on sandy soil. Agr Ecosyst Environ 65:1-13. doi:10.1016/S0167-8809(97)00043-1  

27. Qiu NW, Lu QT, Lu CM (2003) Photosynthesis, photosystem II efficiency and the xanthophyll cycle in the salt-adapted halophyte Atriplex centralasiatica. New Phytol 159:479-486. doi:10.1046/j.1469-8137.2003.00825.x  

28. Rodriguez H, Fraga R (1999) Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol Adv 17:319-339. doi:10.1016/S0734-9750(99)00014-2   

29. Rojas-Tapias D, Moreno-Galván A, Pardo-Díaz S, Obando M, Rivera D, Bonilla R (2012) Effect of inoculation with plant growthpromoting bacteria (PGPB) on amelioration of saline stress in maize (Zea mays). Appl Soil Ecol 61:264-272. doi:10.1016/ j.apsoil.2012.01.006  

30. Ryu JH, Yang CH, Kim TK, Lee SB, Kim S, Choi WY, Baek NH, Kim SJ, Chung DY (2010) Elution patterns and distribution of salts from multi-layer reclaimed soils with subsurface layer of porous granules in the newly reclaimed Saemangeum tidal area. 19th World Congress of Soil Science, Soil Solutions for a Changing World, Brisbane, Australia, pp 121-123  

31. Salazar-Henao JE, Vé lez-Bermúdez IC, Schmidt W (2016) The regulation and plasticity of root hair patterning and morphogenesis.Development 143:1848-1858. doi:10.1242/dev.132845  

32. Sarwar M, Arshad M, Martens DA, Frankenberger Jr WT (1992) Tryptophan-dependent biosynthesis of auxins in soil. Plant Soil 147:207-215. doi:10.1007/BF00029072  

33. Sohn YM, Jeon GY, Song JD, Lee JH, Kim DH, Park ME (2010) Effect of soil salinity and soil-wetting by summer-rising of water table on the growth of fruit trees transplanted at the Saemangeum reclaimed tidal land in Korea. Korean J Soil Sci Fert 43:8-15   

34. Suzuki S, He Y, Oyaizu H (2003) Indole-3-Acetic acid production in HP72 and its association with suppression of creeping bentgrass brown patch. Curr Microbiol 47:138-143. doi:10.1007/s00284-002-3968-2   

35. Vazquez P, Holguin G, Puente ME, Lopez-Cortes A, Bashan Y (2000) Phosphate solubilizing microorganisms associated with the rhizosphere of mangroves growing in a semiarid coastal lagoon. Biol Fert Soils 30:460-468. doi:10.1007/s003740050024   

36. Yue H, Mo W, Li C, Zheng Y, Li H (2007) The salt stress relief and growth promotion effect of Rs-5 on cotton. Plant soil 297:139-145.doi:10.1007/s11104-007-9327-0