Article | 06. 2016 Vol. 34, Issue. 3
Confirmation of Parentage of the Pear Cultivar ‘Niitaka’ (Pyrus pyrifolia) Based on Self-incompatibility Haplotypes and Genotyping with SSR Markers

Department of Horticulture, Sunchon National University1

2016.06. 453:460


The parentage of the horticulturally important pear cultivar ‘Niitaka’ was confirmed by determining its S -genotypes based on the S-RNase and PpSFBB genes, and genotyping using simple sequence repeat (SSR) markers. Previous reports suggested that the cultivars ‘Amanogawa’ and ‘Imamuraaki’ were the parents of ‘Niitaka’, although the cultivars ‘Chojuro’ and ‘Shinchu’ were also examined as candidate parents, along with two other cultivars. In the present study, the S -genotype of ‘Niitaka’ was determined to be S3S9. The S9-RNase of ‘Niitaka’ was found to be likely inherited from the parent ‘Amanogawa’ (S1S9) and the S3-RNase from ‘Chojuro’ (S3S5) or ‘Shinchu’ (S3S5). Based on the S-genotypes, the cultivar ‘Imamuraaki’ (S1S6) had no contribution to the parentage of ‘Niitaka’ (S3S9). A total of 67 polymorphic SSR markers were used to further confirm the parentage of ‘Niitaka’. Discrepancies were found at several SSR loci between ‘Niitaka’ and the cultivars ‘Imamuraaki’ and ‘Shinchu’, whereas ‘Niitaka’ inherited alleles from ‘Amanogawa’ and ‘Chojuro’ at all SSR loci. Therefore, our findings established that ‘Amanogawa’ and ‘Chojuro’ are the parents of pear cultivar ‘Niitaka’, and not ‘Imamuraaki’ as previously reported.

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