Article | 02. 2016 Vol. 34, Issue. 1
Scent Analysis Using an Electronic Nose and Flowering Period of Potted Diploid and Tetraploid Cymbidium

Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration1
Department of Horticultural Science, Chungbuk National2

2016.02. 163:171


We investigated the intensity and pattern of the scent produced by diploid and tetraploid Cymbidium flowers, using an electronic nose with 6 metal oxide sensors (MOS). The MOS responses were evaluated by principal component analysis, discriminant function analysis, and sensor data. These analyses revealed that tetraploid flowers had a stronger scent than diploid flowers in Cymbidium Golden Elf ‘Sundust’. Furthermore, among the different flower partscolumn, lip, and petals-the column produced the strongest scent. There was no significant difference between the flowering periods of diploid and tetraploid potted Cymbidium Golden Elf ‘Sundust' and Cymbidium Elma ‘Orient Toyo’ grown in a greenhouse. Moreover, there were no significant differences between the number of flowers per flower stem and the length of flower stems on the diploid and tetraploid plants of these two Cymbidium cultivars. This study provides potentially useful information for the breeding of polyploidy Cymbidium in the floriculture industry.

1. Alpha M.O.S. 1998. Intelligent electronic nose. FOX user manual. 2nd ed. SA, France. 1:25-31.  

2. Byun, M.S., M.J. Kim, M.K. Kwon, Y.Y. Han, and K.W. Kim. 2007. Characteristic comparison of the flower scent in different flowering stages and floral organs of Lilium using an electronic nose. Flower Res. J. 15:1-8.  

3. Dudareva, N. and E. Pichersky. 2006. Biology of floral scent, Taylor & Francis Group. Boca Raton. FL, USA. p. 346.  

4. Gardner, H.W., D.L., Dornbos Jr., and A.E., Desjardins. 1990. Hexanal, trans-2-hexenal, and trans-2-nonenal inhibit soybean, Glycine max, seed germination. J. Agric. Food Chem. 38:1316-1320.  

5. Gross, K. and F.P. Schiestl. 2015. Are tetraploids more successful? Floral signals, reproductive success and floral isolation in mixedploidy populations of a terrestrial orchid. Ann. Bot. 15: 263-273.  

6. Guterman, I., S.M. Menda, D. Piestun, M. Dafny-Yelin, G. Shalev, E. Bar, O. Davydov, M. Ovadis, M. Emanuel, J. Wang, Z. Adam, E.  

7. Pichersky, E. Lewinsohn, D. Zamir, A. Vainstein, and D. Weiss. 2002. Rose scent: genomics approach to discovering novel floral fragrance–related genes. Plant Cell 14:2325-2338.  

8. Inoue, K., T. Kato, A. Nobukuni, H. Kunitake, and T. Yabuya. 2006. Characterization of tetraploid plants regenerated via protoplast culture of Iris fulva and their crossability with Japanese irises. Sci. Hortic. 10:334-339  

9. Kim, H.S. 2006. Effects of the floral arts program on psychology and brain EEG of middle - aged women. J. Korean Floral Art Res. Assn. 8:56-69.  

10. Kim, M.S., J.Y. Won, Y.R. Lee, J.Y. Kim, B.H. Kim, and J.S. Eun. 1998. Induction of polyploidy in vitro of by colchicine treatment. Korean J. Hortic. Sci. Technol. 16: 115. (Abstr.)  

11. Kim, M.S., J.Y. Kim, and J.S. Eun. 2003. Chromosome doubling of a hybrid with colchicine treatment in meristem culture. Proc. Nagoya Intl. Orchid Congr. Nagoya, Japan. p. 37-40.  

12. Kim, M.S., Y.R. Lee, and J.S. Eun. 2006. Induction of polyploidy of Golden Elf 'Sundust' by colchicine treatment. 27th Intl. Hortic. Cong. Seoul, Korea. p. 157. (Abstr.)  

13. Kim, M.S., J.Y. Kim, and J.S. Eun. 2008. Duplication of the chromosome number of diploid Silky 'Wind' using colchicines. Proc. 1st Asian Hortic. Cong. Jeju, Korea. p. 110-111.(Abstr.)  

14. Lee, K.H., P.H. Park, B.W. Yea, Y.R. Lee, and P.M. Park. 2012. Analysis of flower tissues, collection time, and flowering day in fragrant 'Hwanhee' by electronic nose. Korean J. Hortic. Sci. Technol. 30:154. (Abstr.)  

15. Lee, Y.R., J.W. Chung, and S.R. Kim. 2003. Analysis of fragrance patterns of Rosa hybrida using electronic nose system. Flower Res. J. 11:1-4.  

16. Lee, Y.R., S.R. Choi, B.W. Yae, and D.S. Lee. 2012 Analysis of cut flowers vase life to pot cultivars. Flower Res. J. 20:40-44  

17. Liu, S., S. Chen, Y. Chen, Z. Guan, D. Yin, and F. Chen. 2011. In vitro induced tetraploid of Dendranthema nankingense (Nakai) Tzvel. shows an improved level of abiotic stress tolerance. Sci. Hortic. 27:411-419.  

18. Menninger, E.D. 1963. Diary of a colchicine-induced tetraploid . Am. Orchid Soc. Bull. 32:885-887.  

19. MAF. 2013. Flower cultivation status.(  

20. Noh, B.S., J.W. Ko, S.Y. Kim, and S.J. Kim. 1998. Application of electronic nose in discrimination of the habitat for special agricultural products. Korean J. Food Sci. Technol. 30:1051-1057.  

21. Park, B.H., B.W. Yae, M.S. Kim, Y.R. Lee, P.M. Park, and D.S. Lee. 2011. Fragrance analysis using GC-MS and electronic nose in Phalaenopsis . Flower Res. J. 19:219-224.  

22. Park, H. 1999. Expression mechanism and bioactivity of flower fragrance. Flower Color Fragrance 1:49-68.  

23. Ranney, T.G. 2006. Polyploidy: from evolution to new plant development. Proc. Intl. Plant Prop. Soc. 56:137-142.  

24. Shin, J.A., J.H. Gwon, and K.T. Lee. 2003. Aroma analysis by the electronic nose on red ginseng powder treated with gamma radiation, methyl bromide and phosphine. Korean J. Food Sci. Technol. 35:825-829.  

25. Staples, E.J. 1999. Electronic nose simulation of olfactory response containing 500 orthogonal sensors in 10 seconds. Proc. 1999 IEEE  

26. Tang, Z.Q., D.L. Chen, Z.J. Song, Y.C. He, and D.T. Cai. 2010. In vitro induction and identification of tetraploid plants of Paulownia tomentosa . Plant Cell Tissue Organ Cult. 102:213-220.  

27. Yae, B.W., I.K. Lee, M.S. Kim, P.H. Park, Y.R. Lee, B.N. Jung, K.S. Han, T.J. Kang, and D.S. Lee. 2011. Standard farming handbook: . Rural Development Administration. Suwon. p. 9-185.  

28. Wimber, D.E. and A. Van Cott. 1966. Artificially induced polyploidy in s . Proc. 5th World Orchid Conf. California, USA. p. 27-31.  

29. Wimber, D.E., S. Watrous, and A.J. Mollahan. 1987. Colchicine induced polyploidy in orchids. Proc. 5th World Orchid Conf. California, USA. p. 65-69.