Article | 02. 2016 Vol. 34, Issue. 1
Enhanced Resistance to Sulfur Dioxide Gas in Transgenic Petunia by Stacking both SOD2 and NDPK2 Genes

National Institute of Horticultural & Herbal Science, Rural Development Administration1

2016.02. 154:162


The aim of this study was to develop a transgenic petunia with enhanced resistance to sulfur dioxide (SO2) gas by stacking two genes, SOD2 and NDPK2, which are both known to confer resistance to abiotic stresses. The first-generation hybrids (TF1) were obtained through reciprocal crosses between an SOD2-transgenic line SOD2-2-1-1-35(T4)[S(T4)] and an NDPK2- transgenic line NDPK2-7-1(T2)[N7-1(T2)]. Approximately 32.1-73.0% of the first-generation hybrids (TF1) carried both SOD2 and NDPK2 genes. These hybrids showed 2.6 and 5.1 times less damage than hybrids carrying only SOD2 or NDPK2 genes, respectively, when they were treated with SO2 gas at 30 ppm. This confirmed that the heterozygous hybrids were more resistant to SO2 than the hybrids carrying either one of the resistance genes. Second-generation hybrids (TF2) were obtained by selfing the TF1 individuals. We confirmed the expression of the stacked genes in the TF2 hybrids by phenotypic observation of their response to SO2 gas at 30 ppm as well as using RT-qPCR and photosynthetic efficiency.

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