Article | 02. 2016 Vol. 34, Issue. 1
Enhanced Resistance to Sulfur Dioxide Gas in Transgenic Petunia by Stacking both SOD2 and NDPK2 Genes



National Institute of Horticultural & Herbal Science, Rural Development Administration1




2016.02. 154:162


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The aim of this study was to develop a transgenic petunia with enhanced resistance to sulfur dioxide (SO2) gas by stacking two genes, SOD2 and NDPK2, which are both known to confer resistance to abiotic stresses. The first-generation hybrids (TF1) were obtained through reciprocal crosses between an SOD2-transgenic line SOD2-2-1-1-35(T4)[S(T4)] and an NDPK2- transgenic line NDPK2-7-1(T2)[N7-1(T2)]. Approximately 32.1-73.0% of the first-generation hybrids (TF1) carried both SOD2 and NDPK2 genes. These hybrids showed 2.6 and 5.1 times less damage than hybrids carrying only SOD2 or NDPK2 genes, respectively, when they were treated with SO2 gas at 30 ppm. This confirmed that the heterozygous hybrids were more resistant to SO2 than the hybrids carrying either one of the resistance genes. Second-generation hybrids (TF2) were obtained by selfing the TF1 individuals. We confirmed the expression of the stacked genes in the TF2 hybrids by phenotypic observation of their response to SO2 gas at 30 ppm as well as using RT-qPCR and photosynthetic efficiency.



1. Bjorkman O. and B. Demmig. 1987. Photon yield of O2 evolution and chlorophyll fluorescence at 77K among vascular plants od diverse origins. Planta 170:489-504.  

2. Chan Y.L., K.H. Lin, Sanjaya, L.J. Liao, W.H. Chen, and M.T. Chan. 2005. Gene stacking in Phalaenopsis orchid enhances dual tolerance to pathogen attack. Transgenic Res. 14:279-288.  

3. Francois, I.E.J.A., W.F. Broekaert, and B.P.A. Cammue. 2002. Different approaches for multi-transgene-stacking in plants. Plant Sci. 163:281-295.  

4. Gao X.H., Z.H. Ren, Y.X. Zhao, and H. Zhang. 2003. Overexpression of increases salt tolerance of Arabidopsis. Plant Physiol. 133:1873-1881  

5. Halpin C. 2005 gene stacking in transgenic plants-the challenge for 21st century plant biotechnology. Plant Biotechnol. J. 3:141-155.  

6. Kalunke R.M., M. Janni, S. Bebedettelli, and R. D'Ovidio. 2013. Using biolistics and hybridization to combine multiple glycosidase inhibitor transgenes in wheat. Euphytica 194:443-457.  

7. Kim Y.H., S. Lim, S.H. Han, J.J. Lee, K.J. Nam, J.C. Jeong, H.S. Lee, and S.S. Kwak. 2015. Expression of both CuZnSOD and APX in chloroplasts enhances tolerance to sulfur dioxide in transgenic sweet potato plants. C. R. Biol. 338:307-313.  

8. Lee S.Y., B.H. Han, E.W. Noh, and S.S. Kwak. 2009a. Transferase of or NDP kinase 2 genes into purebred lines of petunia. J. Plant Biotechnol. 36:144-148.  

9. Lee S.Y., J.L. Kim, and D.Y. Kim. 2011. Identification of enhanced resistance to abiotic stress induced by methyl viologen in progeny from a cross of transgenic lines of Petunia . Flower Res. J. 19:269-273.  

10. Lee S.Y., J. L. Lee, J.H. Kim, and K.J. Kim. 2015. Enhanced removal of exogenous formaldehyde gas by AtFALDH -transgenic Petunia. Hortic. Environ. Biotechnol. 56:247-254.  

11. Lee Y.R. S.Y. Choi, O.K. Kwon, and E.J. Huh. 2009b. Injury symptoms of orchids by sulfur dioxide gases in greenhouse. Flower Res. J. 17:214-241.  

12. Ministry of Agriculture, Food and Rural Affairs. 2014. 2013 Statistics of floriculture crops. p. 12-15.  

13. Nakamura M, M. Kuramata, I. Kasugai, M. Abe, and S. Youssefian. 2008. Increased thiol biosynthesis of transgenic poplar expressing a wheat O-acetylserine(thiol) lyrase enhances resistance to hydrogen sulfide and sulfur dioxide toxicity. Plant Cell Rep. 28:313-323.  

14. Nian H.J., D.J. Zhang, Z.D. Zeng, J.P. Yan, K.Z. Li, and L.M. Chen. 2014. Over-expression of heat shock factor gene (AtHsfA1d) from Arabidopsis thaliana confers formaldehyde tolerance in tobacco. Acta Physiol. Plant. 36:1455-1462.  

15. Su X.H., P. Zhou, R. Wang, Z.P. Luo, and Z.L. Xia. 2015. Overexpression of the maize psbA gene enhances sulfur dioxide tolerance in transgenic tobacco. Plant Cell Tissue Organ Cult. 120:303-311  

16. Tang L., S.Y. Kwon, D.J. Yun, S.S. Kwak, and H.S. Lee. 2004. Selection of transgenic potato plants expressing NDP Kinase 2 gene with enhanced tolerance to oxidative stress. Korean J. Plant Biotechnol. 31:191-195.   

17. Tseng M.J., C.W. Liu, and J.C. Yiu. 2008. Tolerance to sulfur dioxide in transgenic Chinese cabbage transformed with both the superoxide dismutase containing manganese and catalase gene of Escherichia coli . Sci. Hortic. 115:101-110.  

18. United States Department of Agriculture. 2011. Floriculture crops 2010 summary. p. 12-23.  

19. Zhao F.Y., S.L. Guo, H. Zhang, and Y.X. Zhao. 2006. Expression of yeast in transgenic rice results in increased salt tolerance.