Article | 12. 2015 Vol. 33, Issue. 6
Development of an Efficient In Vitro Screening Method for Selection of Resistant Lily Cultivars Against Fusarium oxysporum f. sp. lilii



Sustainable Resources Research Center, Korea Research Institute of Bioscience and Biotechnology1
Department of Horticulture, Chungnam National University2




2015.12. 883:890


PDF XML




The soilborne fungus Fusarium oxysporum f. sp. lilii (Fol) is a serious threat to all lily cultivars, especially infecting bulbs and flowers. It has become increasingly important to develop varieties resistant against the bulb rot disease. Genetic diversity of cultivars and reliable screening methods are required for this purpose. Here, an efficient in vitro screening system for evaluating resistance to Fol in 38 in vitro-grown lily plants was investigated. Various factors including culture conditions of Fol, inoculum density, appropriate plant materials, inoculation method and duration, and incubation period of plant materials after inoculation were combined to optimize the screening method. As a result, we suggest optimal conditions for an in vitro screening system for the selection of Fol-resistant lily cultivars as follows. Fol was grown on potato dextrose agar (PDA) medium for 6 days at 25°C in darkness and used as working inoculation. Spore suspensions were prepared (inoculum density: 1.0 × 104 spores‧mL-1), and then leaf segments 1.5 × 2.0 cm2 were inoculated by dipping for 22 hours at 25°C in dark. Later, leaves were cultured on 0.6% agar plates at 25°C and 50% humidity with a photoperiod of 16 hours light/8 hours dark (fluence rate of 40 µmol・m-2・s-1) to examine the progress of bulb rot. After 7 days, disease levels were classified into indices 1 (no symptom) to 6 (serious bulb rot). Soil inoculation of Fol carried out with resistant or susceptible lily cultivars that had been selected through in vitro screening confirmed the reproducibility of results. Therefore, the in vitro screening method established in this study is efficient and reliable for selection of lily cultivars resistant against bulb rot disease.



1. Bald, J.G., A.O. Paulus, and J.V. Lenz. 1983. Control of field root and bulb diseases of eater lily. Plant Dis. 67:1167-1172.  

2. Ham, S.S., E.M. Lee, S.G. Yu, and S.Y. Oh. 2006. Identification of pathogens associated with bulb rot of lily during storage and effects of bulb disinfection on development of lily bulb rot. Plant Dis. 12:20-24.  

3. Jung, S.M., G.C. Song, H.K. Yun, J.B. Kim, K.B. Ma, and S.J. Park. 2008. Influential factor for identifying levels of resistance and in vitro screening system against gray mold in grapevines. Korean J. Hortic. Sci. Technol. 26:432-439.  

4. Jung, Y.H., H. Joung, H.S. Kim, J.H. Jeon, and K.H. Choi. 1996. Detection of lily symptomless virus using RT-PCR technique. Korean J. Plant Pathol. 12:187-190.  

5. Komada, H. 1975. Development of a selective medium for quantitative isolation of Fusarium oxysporum from natural soil. Rev. Plant Prot. Res. 8:114-124.  

6. Lebiush-Mordechai, S., E. Palevsky, L. Tsror, M. Maymon, O. Erlich, S. Freeman, T. Ben-David, and T. Ofek. 2014. Bulb and root rot in lily (Lilium longiflorum) and onion (Allium cepa) in Israel. J. Phytopathol. 162:466-471.  

7. Lee, Y.H., H.W. Choi, I.S. Myung, J.W. Park, K.S. Park, M.J. Lee, S.T. Kim, and S.W. Lee. 2011. Development of in vitro seedling screening method for selection of resistant rice against bakabae disease. Res. Plant Dis. 17:288-294.  

8. Loffler, H.J.M., J.R. Mouris, R.P. Baayen, and T.P. Straathof. 1995. Durability of resistance in lily to basal rot: evaluation of virulence and aggressiveness among isolates of Fusarium oxysporum f.sp. lilii. Eur. J. Plant Pathol.101:261-271.  

9. Loffler, H.J.M. and P. Rumine. 1991. Virulence and vegetative compatibility of Dutch and Italian isolates of Fusarium oxysporum f. sp. lilii. J. Phytopathol. 132:12-20.  

10. Ministry of Agriculture, Food and Rural Affairs (MAFRA). 2014. 2013 Annual reports of flower cultivation status. http://library. mafra.go.kr/skyblueimage/14503.pdf.  

11. Prados-Ligero, A.M., M.J. Basallote-Ureba and J.M. Melero-Vara. 2008. First report of Fusarium oxysporum f. sp. lilii and F. proliferatum affecting Lilium crops in Spain. Trop. Plant Pathol. 33:235-236.  

12. Senthil, M., M.L. Jeeva, R.S. Misra, V.M. Hegde, and V.S. Nath. 2013. In vitro standardization of resistance screening methods in cassava against tuber rot disease. Arch. Phytopathol. Plant Prot. 46:1255-1261.  

13. Straathof, T.P., H.J.M. Loffler, and J. Jansen. 1993. Determination of resistance to Fusarium oxysporum in Lilium. Phytopathol. 83:568-572.  

14. Straathof, T.P. and J.M.Van Tuyl. 1994. Genetic variation in resistance to Fusarium oxysporum f. sp. lilii in the genus Lilium. Ann. Appl. Biol. 125:61-72.