Highland Agricultural Research Institute, National Institute of Crop Science, RDA1
Department of Bio-Health Technology, Kangwon National University2
Department of Plant Science, Gangneung-Wonju National University3
This study was carried out treatment of polyethylene glycol (PEG) in order to increase of survival rate of acclimated plants of strawberry’s in vitro plantlets through bioreactor culture. We used PEG with molecular weight 6000 (PEG 6000) in this study. Concentration of PEG is non-treatment, 5, 10, 15, and 20 g･L-1 each bioreactor. 5 g･L-1 of PEG was treated at 1st, 2nd, 3rd, 4th, and 5th week during culture. We investigated growth characteristics of in vitro plantlets after 6 weeks cultivation. Growth amount of all PEG treatment decreased as compared to non-treatment. The more concentration increased, the more plant growth decreased. In 5 g･L-1 of PEG, shoot length was shorter than non-treatment that shoot length was 7.9 cm and especially fresh weight that is 1.6 g was more decrease than non-treatment. Shoot length was ranged 3.0-3.9 cm at 1st week treatment to 4th week treatment of 5 g･L-1 PEG. The shoot length was not significant by 5.3 cm at 5th week treatment. The survival rate was improved 5.4% at the treatment of 4th week and was improved 8.7% at the 5th week as compared to non-treatment. In order to improve of survival rate of strawberry’ in vitro plantlets through bioreactor culture, the method is suitable that adding 5 g･L-1 of PEG in the medium and 5th week’s treatment is suitable.
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