Article | 08. 2015 Vol. 33, Issue. 4
Optimalization of Flurprimidol Concentration for Improvement of Acclimatization Rate and In Vitro Growth Inhibition in Bioreactor Culture of Strawberry Plantlets via Bioreactor Culture



Highland Agricultural Research Center, National Institute of Crop Science, RDA1
Department of Bio-Health Technology, Kangwon National University2
Department of Plant Science, Gangneung-Wonju National University3




2015.08. 585:590


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This study was conducted to improve the acclimatization rate of in vitro strawberry plantlets through bioreactor culture using the growth retardant flurprimidol. Different concentrations [0 (Control), 0.1, 0.5, 1.0, and 2.0 mg・L-1] of flurprimidol were added during bioreactor culture. After six weeks of treatments, various growth characteristics were investigated and in vitro plantlets were acclimated in the greenhouse. The growth rate of treated plantlets was much lower than that of control, and as the treatment concentration increased, the growth rate was much decreased. Shoots of plantlets treated with flurprimidol were shorter (2.2-3.7 cm) than those of control (7.9 cm). The number of roots per treated plant was around 11.6-34.2, compared with 51.8 in the control. Root length was also lower (0.88-3.08 cm) than control (4.36 cm). However, the number of new shoots and leaves increased in all treatments except for 2.0 mg・L-1 concentration. The root was partially decayed in 1.0 mg・L-1 concentration and was completely decayed in 2.0 mg・L-1. The survival rate in 0.1 mg・L-1 and 0.5 mg・L-1 concentrations was 100% and 23.3% respectively. After four weeks of acclimatization, the plantlets restarted growth, and growth characteristics of shoots and roots recovered to the levels of control, except for fresh weight. Based on our results, a concentration of 0.1 mg・L-1 flurprimidol is appropriate for improvement of acclimatization rate of in vitro strawberry plantlets in bioreactor culture.



1. Berruti, A. and V. Scariot. 2013. Efficacy of flurprimidol and peat alternatives on growth control of potted camellias. N. Z. J. Crop Hortic. Sci. 41:230-239.  

2. Chen, J. and M. Ziv. 2001a. Ancymidol effect on oxidative stress and the regeneration potential of Narcissus leaves in liquid culture. Acta Hortic. 560:299-302.  

3. Chen, J. and M. Ziv. 2001b. The effect of ancymidol on hyper-hydricity, regeneration, starch and antioxidant enzymatic activities in liquid-cultured Narcissus. Plant Cell Rep. 20:22-27.  

4. Ellington, E., T. Adserias, A. Coma, J. Bastida, F. Viladomat, and C. Codina. 1997. Effect of paclobutrazol on in vitro culture of Colchicum autumnale corms. Acta hortic. 447:131-133.  

5. Graebe, J.E. 1987. Gibberellin biosynthesis and control. Annu. Rev. Plant Physiol. 381:419-465.  

6. Guen-Le Saos, F., A. Hourmant, F. Esnault, and J.E. Chauvin. 2002. In vitro bulb development in schaliot (Allium cepa L. Aggregatum Group): effects of anti-gibberellins, sucrose and light. Ann. Bot. 89:419-425.  

7. Hazarika, B.N. 2003. Acclimatization of tissue -cultured plants. Current Sci. 85:1704-1712.  

8. Ilczuk, A., T. Winkelmann, S. Richartz, M. Witomska, and A. Serek. 2005. In vitro propagation of Hippeastrum × chmielii Chm. - influence of flurprimidol and the culture in solid or liquid medium and in temporary immersion systems. Plant Cell, Tissue Organ Cult. 83:339-346.  

9. Kim, E.K., E.J. Hahn, H.N. Murthy, and K.Y. Paek. 2003. High frequency of shoot multiplication and bulblet formation of garlic in liquid cultures. Plant Cell, Tissue Organ Cult. 73:231-236.  

10. Kim, H.J., J.N. Lee, K.D. Kim, J.S. Im, H.T. Lim, and Y.R. Yeoung. 2011. Suitable hormone-free medium for in vitro mass propagation via bioreactor culture of ever-bearing strawberry. J. Plant Biotechnol. 38:221-227.  

11. Lee, J.N., H.J. Kim, K.D. Kim, Y.S. Kwon, J.S. Im, H.K. Lim, and Y.R. Yeoung. 2010. In vitro mass propagation and economic effects of bioreactor culture in ever-bearing strawberry ‘Goha’. Kor. J. Hort. Sci. Technol. 28:845-849.  

12. Lee, J.N., H.J. Kim, K.D. Kim, J.S. Im, H.T. Lim, and Y.R. Yeoung. 2012. Growth characteristics and economic efficiency of nursery plants production according to transplanting container for acclimatization of mass propagated plantlets via bioreactor culture of ever-bearing strawberry ‘Goha’. Kor. J. Hort. Sci. Technol. 30:437-441.  

13. McClelland, M.T. and M.A.L. Smith. 1990. Vessel type, closure, and explants orientation influence in vitro performance of five woody species. Hortic. Sci. 25:797-800.  

14. Sansavini, S. P. Rosati, D. Gaggioli, and M.F. Toshi. 1990. Inheritance and stability of somaclonal variation in micro-propagated strawberry. Acta Hortic. 280:375-384.  

15. Smith, E.F., A.V. Roberts, J. Mottley, and S. Denness. 1991. The preparation in vitro of chrysanthemum for transplantation to soil. 4. The effect of eleven growth retardants on wilting. Plant Cell, Tissue Organ Cult. 27:309-313.  

16. Sutter, E.G. 1988. Stomata and cuticular water loss from apple, cherry, and sweet-gum plants after removal from in vitro culture. J. Am. Soc. Hortic. Sci. 113:234-238.  

17. Sutter, E.G. and R.W. Langhans. 1979. Epicuticular wax formation on Carnation plantlets regenerated from shoot-tip culture. J. Am. Soc. Hortic. Sci. 104:493-496.  

18. Wardle, K., E.B. Dobbs, and K.L. Short. 1983. In vitro acclimati-zation of aseptically cultured plantlets to humidity. J. Am. Soc. Hortic. Sci. 108:386-389.  

19. Ziv, M. 1989. Enhanced shoot and cormlet proliferation in liquid cultured Gladiolus buds by growth retardants. Plant Cell, Tissue Organ Cult. 17:101-110.  

20. Ziv, M. 1990. The effect of growth retardants on shoot proliferation and morphogenesis in liquid cultured Gladiolus plants. Acta Hortic. 280:247-258.