Article | 08. 2015 Vol. 33, Issue. 4
Linkage Analysis of the Three Loci Determining Rind Color and Stripe Pattern in Watermelon

NH Seed Research & Development Center1
Department of Horticultural Bioscience, Pusan National University2
Department of Integrative Plant Science, Chung-Ang University3
Department of Life Science and Biotechnology, ShinGyeong University4

2015.08. 559:565


The rind phenotype of watermelon fruits is an important agronomic characteristic in the watermelon market. Inheritance and linkage analyses were performed for three rind-related traits that together determine the rind phenotype: foreground stripe pattern, rind background color, and depth of rind color. The inheritance of the foreground stripe pattern was analyzed using three different F2 populations, showing that the striped pattern is dominant over the non-striped pattern. The inheritance analysis of the rind background color was performed using F2 populations of the ‘10909’ and ‘109905’, and the depth of rind color was analyzed using F2 populations of the ‘90509’ and ‘109905’. Yellow color was found to be dominant over green color, and a deep color was dominant over the standard color. Linkage analysis of the three traits was conducted using three F2 populations in which two traits were segregating. Each pair of traits was inherited independently, which demonstrated that the three traits are not linked. Therefore, we propose a three-locus model for the determination of rind phenotype, providing novel insight that rind phenotype is determined by the combination of three genetically independent loci.

1. Barham, W.S. 1956. A study of the Royal Golden watermelon with emphasis on the inheritance of the chlorotic condition characteristic of this variety. Proc. Am. Soc. Hortic. Sci. 67: 487-489.  

2. Guner, N. and T.C. Wehner. 2004. The genes of watermelon. HortScience 39:1175-1182.  

3. Gusmini, G. and T.C. Wehner. 2006. Qualitative inheritance of rind pattern and flesh color in watermelon. J. Hered. 97:177-185.  

4. Henderson, W.R. 1991. Gene list for watermelon. Cucurbit Genet. Coop. Rep. 14:129-138.  

5. Henderson, W.R. 1992. Corrigenda to 1991 watermelon gene list (CGC14:129-138). Cucurbit Genet. Coop. Rep. 15:110.  

6. Kumar, R. and T.C. Wehner. 2011. Discovery of second gene for solid dark green versus light green rind pattern in watermelon. J. Hered. 102:489-493.  

7. Rhodes, B. and F. Dane. 1999. Gene list for watermelon. Cucurbit Genet. Coop. Rep. 22:61-77.  

8. Rhodes, B. and X. Zhang. 1995. Gene list for watermelon. Cucurbit Genet. Coop. Rep. 18:69-84.  

9. Poole, C.F., P.C. Grimball, and D.R. Porter. 1941. Inheritance of seed characters in watermelon. J. Agric. Res. 63:433-456.  

10. Poole, C.F. 1944. Genetics of cultivated cucurbits. J. Hered. 35: 122-128.  

11. Porter, D.R. 1933. Watermelon breeding. Hilgardia 7:585-624.  

12. Porter, D.R. 1937. Inheritance of certain fruit and seed characters in watermelons. Hilgardia 10:489-509.  

13. Weetman, L.M. 1937. Inheritance and correlation of shape, size, and color in watermelon, Citrullus vulgaris Schrad. Iowa Agric. Exp. Stn. Annu. Bull. 228:224-256.   

14. Wehner, T.C. 2008. Watermelons, p. 381-418. In: Prohens J. and Nuez F. (eds). Handbook of plant breeding. Vegetables I: Asteraceae, Brassicaceae, Chenopodiaceae, and Cucurbitaceae. Springer Science Business LLC. New York. USA.