Article | 06. 2015 Vol. 33, Issue. 3
Occurrence and Analysis of Apple Blotch-like Symptoms on Apple Leaves

School of Applied Biosciences, Kyungpook National University1
Department of Horticultural Science, Kyungpook National University2
Institute of Plant Medicine, Kyungpook National University3
Horticultural and Herbal Crop Environment Division, National Institute of Horticultur and Herbal Science (RDA)4

2015.06. 429:434


Apple blotch-like symptoms (ABLS) were observed on ‘Fuji’ apple leaves in Cheongsong, Gunwi and Yeongcheon apple orchards located in Gyeongbuk Province during 2010-2014. Characteristics of ABLS were yellowing, brown spots on leaves, and defoliation, similar to apple blotch diseased (ABD) leaves, which are infected with Marssonina coronaria. It is difficult to differentiate by eye between ABLS and ABD, which has led to misdiagnosis and overuse of fungicides. The present study was conducted to investigate the cause of ABLS using stereomicroscopy, culture isolation, cross-sectional analysis of leaves, and PCR. No acervuli were found on the surface of ABLS leaves and no growth was observed on potato dextrose agar (PDA) plates in culture. Furthermore, cross-sectional analysis revealed similar results, and mycelia were absent in ABLS leaves. By contrast, all these characteristics were present in ABD leaves. Furthermore, no fungi or viruses were detected in ABLS leaves by PCR, suggesting that the disease is not caused by these agents. These findings suggest that ABLS might be a physiological disorder in plants that is distinct from ABD.

1. Babadoost, M. 1984. Necrotic leaf blotch of Golden Delicious apples. Report on plant disease. RPD No.817.  

2. Back, C.G. and H.Y. Jung. 2014. Biological characterization of Marssonina coronaria infecting apple trees in Korea. Kor. J. Mycol. 42:183-190.  

3. Ellis, M.A. 2008. Necrotic leaf blotch of Golden Delicious apples. The Ohio State University Extension. Fact Sheet HYG. p. 3204-3208.  

4. Gianni, T., S. Yasuo, L.U. Sandie, L. Bruce, O. Nichole, K.H. William, and T.M. DeJong. 2006. Silencing leaf sorbitol synthesis alters long-distance partitioning and apple fruit quality. Proc. Natl. Acad. Sci. doi:10.1073/pnas.0605873103.  

5. Hassan, M., A. Myrta, and J. Polak. 2006. Simultaneous detection and identification of four pome fruit viruses by one tube pentaplex RT-PCR. J. Virol. Methods 133:124-129.  

6. Jonkers, H. 1973. Review on leaf spot and leaf drop: A physiological disorder of the ‘Golden Delicious’ apple. Sci. Hortic. 1:231-237.  

7. Kender, W.J. and F.H.F.G. Spierings. 1975. Effects of sulfur dioxide, ozone, and their interactions on ‘Golden Delicious’ apple trees. Neth. J. Plant Pathol. 81:149-151.  

8. Kim, W.G., H.M. Koo, K.H. Kim, I.H. Hyun, S.K. Hong, J.S. Cha, Y.K. Lee, K.H. Kim, H.S. Choi, and D.G. Kim. 2009. List of plant diseases in Korea. 5th ed. Anyang: Kor. Soc. Plant Pathol.   

9. Lee, D.H., C.G. Back, N.K.K. Win, K.H. Choi, K.M. Kim, I.K. Kang, C. Choi, T.M. Yoon, J.Y. Uhm, and H.Y. Jung. 2011. Biological characterization of Marssonina coronaria associated with apple blotch disease. Mycobiology 39:200-205.   

10. Lee, D.H., H.C. Shin, R.H. Cho, and J.Y. Uhm. 2009. Reducing fungicidal spray frequency for major apple diseases by increasing thespray interval from 15 to 25 days. Plant Pathol. J. 25:270-279.  

11. Lee, H.T. and H.D. Shin. 2000. Taxonomic studies on the genus Marssonina in Korea. Mycobiology 28:39-46.  

12. Lee, J.H., J. K. Park, D.H. Lee, J.Y. Uhm, S.Y. Ghim, and J.Y. Lee. 2001. Occurrence of apple scar skin viroid-Korean strain (ASSVd-K) in apples cultivated in Korea. Plant Pathol. J. 17:300-304.   

13. Lee, S.W., D.H. Lee, K.H. Choi, and D.A. Kim. 2007. A report on current management of major apple pests based on census data from farmers. Kor. J. Hort. Sci. Technol. 25:196-203.  

14. Menzel, N., W. Jelkmann, and E. Maiss. 2002. Detection of four apple viruses by multiplex RT-PCR assays with coamplification of plant m-RNA as internal control. J. Virol. Methods 99:89-92.   

15. Namba, S., S. Kato, S.Iwanamis, H. Oyaizu, H. Shiozawa, and T. Tsuchizaki. 1993. Detection and differentiation of plant-pathogenic mycoplasma-like organism using polymerase chain reaction. Phytopathology 83:786-791.  

16. Rosenberger, D.A. 2001. Leaf spotting: causes and solutions. Proc. New England Fruit Growers Mtg., Sturbridge, USA.  

17. Sharma, J.N. 2000. Marssonina blotch – a new disease of apple and its control. Indian J. Plant Prot. 28:100-101.  

18. Shim, H.K., H.K. Hwang, C.K. Shim, S.W. Son, D.G. Kim, and S.C. Lee. 2006. The pear black necrotic leaf spot disease virus transmitted by Talaramyces flavus displays pathogenicity similar to apple stem  grooving virus strain. Kor. J. Plant Pathol. 22:255-259.  

19. Sutton, T.B. and C.N. Clayton. 1974. Necrotic leaf blotch of Golden Delicious apples. NC Agric. Exp. Bull. 224.  

20. Sutton, T.B. and R.M.V. Sanhueza.1998. Necrotic leaf blotch of Golden Delicious-Glomerella leaf spot: a resolution of common names. Plant Dis. 82:267-268.   

21. Tamietti, G. and A. Matta.2003. First report of leaf blotch caused by Marssonina coronaria on apple in Italy. Plant Dis. 87:1005.  

22. Yoder, K.S. and A.R. Biggs. 1998. West Virginia University Extension Service Factsheets, WVU Extension Service.