Article | 06. 2015 Vol. 33, Issue. 3
Differential Frost Tolerance and Enzymatic Activities in the Leaves and Immature Fruits of Loquat (Eriobotrya japonica Lindl.)

Horticulture College, Fujian Agriculture and Forestry University, Fuzhou1
Fujian Science Institute of Tropical Crops, Zhangzhou2
College of Life Science, Fujian Agriculture and Forestry University, Fuzhou3
Graham Centre for Agricultural Innovation (NSW Department of Primary Industries and Charles Sturt University), Wagga Wagga Agricultural Institute, PMB4

2015.06. 309:316


In this experiment, five commercial cultivars and one wild species of loquat were used to investigate frost tolerance and enzymatic activities in leaves and young fruits under cold stress at -3°C. The frost injury, malondialdehyde (MDA) content, and oxygen-scavenging enzyme activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) were studied. This results showed that the wild species ‘Wild Oak-leaf’ loquat was the most frost tolerant among accessions tested, followed by the cultivar ‘Golden Block’. Other cultivars, ‘Wu Gong Bai’, ‘Taicheng 4’, ‘Xiangzhong 11’ and ‘Zaozhong 6’, were relatively weak in frost tolerance. The enzymatic activities of SOD, POD and CAT increased initially and then decreased as the exposure time increased. However, the enzymatic peak occurred later in the frost-tolerant accession than in the frost-sensitive accession. The correlation coefficients of MDA contents between leaves and immature fruits were from 0.93 to 0.99 in the five commercial loquat cultivars. For the ‘Wild Oak-leaf’ loquat, the correlation coefficients of MDA and POD were 0.98 and 0.95, respectively, but the coefficients for SOD, CAT and APX were relatively low. In general, there were good correlations between loquat leaves and immature fruits in MDA content and enzyme activities. These results indicate that analysis of these physiological and biochemical activities in loquat leaves could potentially be used to predict the cold tolerance in loquat at immature fruit stage and to accelerate breeding programs for cold tolerance in loquat.

1. Chen, L.S. and L. Cheng. 2003. Both xanthophyll cycle-dependent thermal dissipation and the antioxidant system are up-regulated in grape (Vitis labrusca L. cv. Concord) leaves in response to N limitation. J. Exp. Bot. 54:2165-2175.  

2. Duan, Y., S. Song, and J. Fu. 1998. Effects of calcium on senescence in detached leaves of hybrid rice. Acta Sci. Nat. Univ. Sunyatseni 37:83-87.  

3. Ferreres, F., D. Gomes, P. Valentão, R. Gonçalves, R. Pio, E.A. Chagas, R.M. Seabra, and P.B. Andrade. 2009. Improved loquat (Eriobotrya japonica Lindl.) cultivars: Variation of phenolics and antioxidative potential. Food Chem. 114:1019-1027.  

4. Giannopolitics, C.N. and S.K. Ries. 1977. Superoxide dismutase. 1.Occurrence in higher plants. Plant Physiol. 59:309-314.  

5. Hassanpour, H., R.A. Khavari-Nejad, V. Niknam, F. Najafi, and K. Razavi. 2012. Effects of penconazole and water deficit stress on physiological and antioxidative responses in pennyroyal (Mentha pulegium L.). Acta Physiol. Plant. 34:1537-1549.  

6. Heidari, B., M. Pessarakli, A. Dadkhodaie, and N. Daneshnia. 2012. Reactive oxygen species-mediated functions in plants under environmental stresses. J. Agric. Sci. Technol. B2:159-168.  

7. He, Q., X.W. Li, G.L. Liang, K. Ji, Q.G. Guo, W.M. Yuan, G.Z. Zhou, K.S. Chen, W.E. van de Weg, and Z.S. Gao. 2011. Genetic diversity and identity of Chinese loquat cultivars/accessions (Eriobotrya japonica) using apple SSR markers. Plant Mol. Biol. Rep. 29:197-208.  

8. Hueso, J.J. and J. Cuevas. 2008. Loquat as a crop model for successful deficit irrigation. Irrigation Sci. 26:269-276.  

9. Jiang, R.F., Y.F. Chen, and Y.Q. Lin. 2010. Feature analysis and forecast research of loquat frost injury in Putian. Sci. Technol. West China 9:6-8.  

10. Jiang, W.B., Y.L. Wang, and K. Ma. 1992. Effect of osmo protective substances on cold hardiness of fig. Acta Hortic. Sin. 19:371-372.  

11. Lin, D., Y.J. Yang, W.L. Fan, and Y.T. Wei. 2001. Effects of low temperature on active oxyen metabolism of tomato seedlings leaves. Liaoning Agric. Sci. 5:5-8.  

12. Liu, H.X., S.X. Zeng, Y.R. Wang, P. Li, D.F. Chen, and J.Y. Guo. 1985. The effect of low temperature on superoxide dismutase in various organelles of cucumber seedling cotyledon with different cold tolerance. Acta Phytophysiologica Sinica 11:48-57.  

13. Liu, X.Q., X.F. Wang, and Y.J. Piao. 2007. A Study on cold tolerance of different phalaenopsis cultivars. Acta Hortic. Sin. 34: 425-430.  

14. Liu, Z.Q. and S.C. Zhang. 1994. Plant resistant physiology, p. 371-372. China Agriculture Press, Beijing, China.  

15. Peng, C.C. and Z.H. Sun. 2000. Changes of SOD and CAT activities of citrus protoplast during cold acclimation. J. Huazhong Agric. Uni. 19:384-387.  

16. Rao, M.V., G. Paliyath, and D.P. Ormrod. 1996. Ultraviolet-B-and ozone-induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana. Plant Biol. 110:125-136.  

17. Ren, H.H., W. Huang, and F.M. Zhang. 2002. Effects of low temperature and poor light on some physiological parameters of tomato. J. China Agric. Uni. 7:95-101.  

18. Sun, S.X., J. Li, M.Y. Tu, D. Chen, H.J. Xie, and G.L. Jiang. 2011. Freezing damage factors and physiological indexes of frost resistance in loquat. ISHS Acta Hortic. 887:171-176.  

19. Wang, F., H. Wang, D.W. Chen, and J.R. Li. 1999. A Study on the hardiness of flower organ of Apricot accessions. Acta Hortic. Sin. 26:356-359.  

20. Wang, H., J.P. Peng, S.M. Fang, G.D. Zheng, C.J. Qiu, Q. Huang, Y.S, Chen, and G.H. Zheng. 2008. Relationship between ice nucleation bacteria and frost in loquat. Plant Prot. 34:43-46.  

21. Wang, J.H., H.X. Liu., and T. Xu. 1989. The role of superoxide dismutase (SOD) in stress physiology and senescence physiology of plant. Plant Physiol. Comm. 1:1-7.  

22. Xie, Z. and J. Li. 2006. Definition on Freezing Injury Temperature of ‘Zaozhong 6’ Loquat Young Fruits and Division of Suitable Cultivating Area.Fujian Fruits 1:7-11.  

23. Xuan, J., H. Zhang, and Z. Guo. 2013. Physiological response of zoysiagrass in urban landscapes to low temperature stress. ISHS Acta Hortic. 999:253-261.  

24. Yang, F.J., B.J. Li, and Y.G. Gao. 2003. Recent progress in the study of cold resistance of fruit tree. J. Heilongjiang Aug. First Land Reclam. Univ. 15:23-29 15:23-29.  

25. Yue, H., G.H. Li, G.W. Li, L.L. Chen, G.H. Kong, and G.P. Liang. 2010. Studies on cold resistance of different macadamia cultivars. Acta Hortic. Sin. 37:31-38.  

26. Zhou, B., J. Chen, Z. Ji, and R. Cai. 1999. Changes of superoxide dismutase activity and water-soluble protein content in bananas during winter. J. Fruit Sci. 16:192-196.  

27. Zhou, Z. and G. Lu. 1994. The effect of chilling stress on membrane lipid peroxidation and protective enzyme in pepper seedlings. Acta Agric. Boreali-Occidentalis Sin. 3:51-56.  

28. Zhu, G.L. 1990. Plant Physiology Experiment, p. 38-39. Beijing University Press, Beijing, China.