Article | 02. 2015 Vol. 33, Issue. 1
Biosafety and Toxicological Evaluation of Tissue-Cultured Echinacea purpurea Adventitious Roots

Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University1
Department of Botany, Karnatak University2

2015.02. 124:132


Echinacea purpurea (L.) Moench (purple cone flower) is an important medicinal plant; it can enhance immunity, relieve pain, and reduce inflammation, and also has hormonal, antiviral, and antioxidant effects. Adventitious root biomass of Echinacea purpurea was produced in commercial-scale bioreactors for use as a dietary supplement in the food industry and in traditional medicine. Biosafety and toxicological evaluations of tissue-cultured Echinacea purpurea adventitious roots (TCEPARs) were performed. Reverse mutation and chromosomal aberration tests showed no significant mutagenicity. Furthermore, repeated four-week oral dose tests performed in Sprague-Dawley rats did not show any notable changes in the general behavior of the rats, in the gross appearance of their internal organs, or in their mortality rate. There were no differences between the control group and the treatment group in parameters such as absolute body weight, hematology, blood chemistry, and absolute and relative organ weights. These findings indicate that TCEPARs are safe and nontoxic when consumed at an average dietary level and can be used as raw material for traditional medicine and the food industry.

1. Abbasi, B.H., P.K. Saxena, S.J. Murch, and C.Z. Liu. 2007. Echinacea biotechnology: Challenges and opportunities. In Vitro Cell. Dev. Biol. Plant 43:481-492.   

2. Ames, B., J. McCann, and I.E. Yamasaki. 1975. Methods for detecting carcinogens and mutagens with Salmonella/mammalian microsome mutagenicity test. Mut. Res. 31:347-364.  

3. Bauer, R. and H. Wagner. 1991. Echinacea species as potential immunostimulating drugs, p. 253-321. In: H. Wagner and N.R. Farnsworth (ed.) Economic and medicinal plant research, Vol. 5. Academic press, New York, USA.  

4. Barrett, B. 2003. Medicinal properties of Echinacea: A critical review. Phytomed. 10:66-86.  

5. Blumenthal, M. 2003. The ABC clinical guide to herbs. American Botanical Council, Thieme, New York, USA.  

6. Caillet, S., S. Lessard, G. Lamoureux, and M. Lacroix. 2011. Umu test applied for screening natural antimutagenic agents. Food Chem. 124:229-248.  

7. Jeong, J.A., C.H. Wu, H.N. Murthy, E.J. Hahn, and K.Y. Paek. 2009. Application of airlift bioreactors for the production of adventitious root biomass and caffeic acid derivatives of Echinacea purpurea. Biotechnol. Bioprocess Eng. 14:91-98.  

8. Marriott, B.M. 2003. An introduction to dietary supplements of plant origin, p. 1-17. In: M. Maffi (ed.). Dietary supplements of plant origin, Taylor and Francis, New York, USA.  

9. Mengs, U., C.B. Clare, and J.A. Poilsey. 1991. Toxicity of Echinacea purpurea, acute, subacute, and genotoxicity studies. Drug Res. 41:1076-1081.  

10. Miller, S.C. 2005. Echinacea: A miracle herb against ageing and cancer? Evidence in vivo in mice. Evi. Based Compl. Altern. Med. 2:309-314.  

11. Paek, K.Y., H.N. Murthy, and E.J. Hahn. 2009. Establishment of adventitious root cultures of Echinacea purpurea for the production of caffeic acid derivatives, p. 3-16. In: S.M. Jain and P.K. Saxena (eds.). Methods in molecular biology, protocols for in vitro cultures and secondary metabolite analysis of aromatic and medicinal plants, Vol. 547. Humana Press, New York, USA.  

12. Percival, S.S. 2000. Use of Echinacea in medicine. Biochem. Pharmacol. 60:155-158.  

13. Perri, D., J.J. Dugoua, E. Mills, and G. Koren. 2006. Safety and efficacy of Echinacea (Echinacea angustifolia, E. purpurea and E. pallida) during pregnancy and lactation. Can. J. Clin. Pharmacol. 13:262-267.   

14. Sivakumar, G., K.W. Yu, J.S. Lee, J.K. Kang, H.L. Lee, W.J. Kim, and K.Y. Paek. 2006. Tissue cultured mountain ginseng adventitious roots: Safety and toxicity evaluation. Eng. Life Sci. 6:372-383.   

15. Tsai, Y.L., S.Y. Chiou, K.C. Chan, J.M. Sung, and S.D. Lin. 2012. Caffeic acid derivatives, total phenol, antioxidant and antimutagenic activities of Echinacea purpurea flower extracts. LWT - Food Sci. Technol. 46:169-176.  

16. Wills, R.B.H., K. Bone, and M. Morgan. 2000. Herbal products: Active constituents, mode of action and quality control. Nutr. Res. Rev. 13:47-77.  

17. Wu, C.H., H.N. Murthy, E.J. Hahn, H.L. Lee, and K.Y. Paek. 2008. Efficient extraction of caffeic acid derivatives from adventitious roots of Echinacea purpurea. Czech J. Food Sci. 26:254-258.   

18. Wu, C.H., H.N. Murthy, E.J. Hahn, and K.Y. Paek. 2007. Large scale cultivation of adventitious roots of Echinacea purpurea in airlift bioreactors for the production of chichoric acid, chlorogenic acid and caftaric acid. Biotechnol Lett. 29:1179-1182.  

19. Yen, G.C. and H.Y. Chen. 1995. Antioxidant activity of various tea extracts in relation to their antimutagenicity. J. Agr. Food Chem. 43:27-32.  

20. Yu, H.C. and M. Kaarlas. 2004. Popularity, diversity, and quality of Echinacea, p. 29-52. In: S. Miller (ed.). Echinacea, the genus Echinacea. CRC Press, Boca Raton, Florida, USA.  

21. Zahin, M., F. Aquil, and I. Ahmad. 2010. Broad spectrum anti-mutagenecity activity of antioxidant active fraction of Punica granatum L. peel extracts. Mut. Res. 703:99-107.