Article | 02. 2015 Vol. 33, Issue. 1
Relationship between Endogenous Polyamines and Floral Bud Differentiation in Chrysanthemum morifolium under Short-day Conditions



College of Horticulture Science and Engineering, Shandong Agricultural University1
State Key Laboratory of Crop Biology, Shandong Agricultural University2




2015.02. 31:38


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This study was carried out to investigate the relationship between endogenous polyamines (PAs) and floral bud differentiation in chrysanthemum (Chrysanthemum morifolium). In this study, PA content (free, bound, and conjugated) in apical buds, leaves, and roots changed appreciably during floral bud differentiation. PAs accumulated during series of processes such as floral induction, differentiation of floret primordia, and crown formation in apical buds; changes in PAs in apical buds may have a relationship with those in leaves and roots. The levels of free PAs and conjugated PAs [putrescine (Put) and spermine (Spm)] in apical buds rapidly increased during the initiation stage of floral bud differentiation, while free and conjugated spermidine (Spd) reached their highest levels at the stage of floret primordium differentiation. In the free, conjugated, and bound PA fractions, the changes in Spm content were negligible compared to those of Put and Spd throughout the experiment. These findings indicate that PAs participate in regulating the process of flower bud differentiation in chrysanthemum.



1. Antognoni, F., S. Fornalè, C. Grimmer, E. Komor, and N. Bagni. 1998. Long-distance translocation of polyamines in phloem and xylem of Ricinus communis L. plants. Planta 204:520-527.  

2. Applewhite, P.B., R. Kaur-Sawhney, and A.W. Galston. 2000. A role for spermidine in the bolting and flowering of Arabidopsis. Physiol. Plant. 108:314-320.  

3. Aribaud, M. and J. Martin-Tanguy. 1994. Polyamine metabolism, floral initiation and floral development in chrysanthemum (Chrysanthemum morifolium Ramat.). Plant Growth Regul. 15:23-31.  

4. Bachrach, U. 1973. Function of naturally occurring polyamines. Academic Press, New York, USA.  

5. Bagni, N. and A. Tassoni. 2001. Biosynthesis, oxidation and conjugation of aliphatic polyamines in higher plants. Amino Acids. 20:301-317.  

6. Buchanan, B.B., W. Gruissem, and R.L. Jones. 2000. Biochemistry and molecular biology in plants. American Society of Plant Physiologists. Maryland, USA. p. 1-90.  

7. Cabanne, F., M.A. Dalebroux, J. Martin-Tanguy, and C. Martin. 1981. Hydroxycinnamic acid amides and ripening to flower of Nicotiana tabaccum var. xanthi n.c. Physiol. Plant. 53:399-404.  

8. Caffaro, S.V., F. Antognoni, S. Scaramagli, and N. Bagni. 1994. Polyamine translocation following photoperiodic flowering induction in soybean. Physiol. Plant. 91:251-256.  

9. Caffaro, S.V. and C. Vicente. 1995. Early changes in the content of leaf polyamines during the photoperiodic flowering induction in soybean. J. Plant Physiol. 145:756-758.  

10. Davis, S.J. 2009. Integrating hormones into the floral-transition pathway of Arabidopsis thaliana. Plant Cell Environ. 32:1201-1210.  

11. Dumas, E., E. Perdrizet, and J.C. Vallee. 1981. Quantitative evolution of free amino acids and amines in the development of various species of Nicotiana. Physiol. Veg. 19:155-165.  

12. Feng, F. and J.S. Yang. 2011. Relationship between floral bud differentiation and endogenous hormones in autumn-cutting Chrysanthemum morifolium ‘Jinba’. Scientia Agr. Sinica. 3:552-561.  

13. Fiala, V., M. Lenard, Y. Querou, and E. Jolivet. 1988. Spermidine as molecular marker of floral induction in the Iris hollandica bulb. C.R. Acad. Sci. Paris. 306:579-582.  

14. Hanzawa, Y., T. Takahashi, A.J. Michael, D. Burtin, D. Long, M. Pineiro, G. Goupland, and Y. Komeda. 2000. ACAULIS5, an Arabidopsis gene required for stem elongation, encodes a spermine synthase. EMBO J. 19:4248-4256.  

15. Havelange, A., P. Lejeune, G. Bernier, R. Kaur-Sawhney, and A.W. Galston. 1996. Purtrescine export from leaves in relation to floral transition in Sinapis alba. Physiol. Plant. 96:59-65.  

16. Huang, C.K., B.S. Chang, K.C. Wang, S.J. Her, T.W. Chen, Y.A. Chen, C.L. Cho, L.J. Liao, K.L. Huang, W.S. Chen, and Z.H. Liu. 2004. Changes in polyamine pattern are involved in floral initiation and development in Polianthes tuberosa. J. Plant Physiol. 161:709-713.  

17. Igarashi, K. and K. Kashiwagi, 2010. Modulation of cellular function by polyamines. Intl. J. Biochem. Cell. B. 42:39-51.  

18. Lang, F., C.S. Zheng, C.H. Zhang, and Q.C. Sun. 2008. Change of carbohydrate content in buds and leave of chrysanthemum during floral differentiation. Shandong Agr. Sci. 1:40-42.  

19. Martin-Tanguy, J. 1985. The occurrence and possible functions of hydroxycinnamoyl acid amides in plant. Plant Growth Regul. 3:381-399.  

20. Martin-Tanguy, J. 1997. Conjugated polyamines and reproductive development: Biochemical, molecular and physiological approaches. Physiol. Plant. 100:675-688.  

21. Martin-Tanguy, J. 2001. Metabolism and function of polyamines in plants: recent development (new approaches). Plant Growth Regul. 34:135-148.  

22. Meijón, M., R. Rodríguez, M.J. Cañal, and I. Feito. 2009. Improve-ment of compactness and floral quality in azalea by means of application of plant growth regulators. Sci. Hortic. 119:169-176.  

23. Meijón, M., M.J. Cañal, H. Fernández, A. Rodríguez, B. Fernández, R. Rodríguez, and I. Feito. 2011. Hormonal profile in vegetative and floral buds of azalea: levels of polyamines, gibberellins, and cytokinins. J. Plant Growth Regul. 30:74-82.  

24. Rajam, M.V., R. Kumria, and S. Singh. 2004. Molecular biology and genetic engineering of polyamines in plants. Plant Biotechnol. Mol. Markers 18:60-77.  

25. Sood, S. and P.K. Nagar. 2004. Changes in endogenous polyamines during flower development in two diverse species of rose. Plant Growth Regulat. 44:117-123.  

26. Tarenghi, E. and J. Martin-Tanguy. 1995. Polyamines,floral induction and flower development of strawberry (Fragaria ananassa). Plant Growth Regulat. 17:157-165.  

27. Wada, N., M. Shinozaki, and H. Iwamura. 1994. Flower induction by polyamines and related compounds in seedlings of morning glory (Pharbitis cv. Kidachi). Plant Cell Physiol. 35:469-472.  

28. Wimalasekera, R., F. Tebartz, and G.F.E. Scherer. 2011. Polyamines, polyamine oxidases and nitric oxide in development, abiotic and biotic stresses. Plant Sci. 181:593-603.  

29. Yang, Y.D., J.W. Su, L.W. Qu, W.D. Wang, N.N. Cui, and S.M. Guo. 2006. The preliminary study on the interaction relationship between flower bud differentiation in ‘Jinba’ and environmental factors. Liaoning Agr. Sci. 1:45-46.  

30. Zhou, Q. and B.J. Yu. 2010. Changes in content of free, conjugated and bound polyamines and osmotic adjustment in adaptation of vetiver grass to water deficit. Plant Physiol Biochem. 48: 417-425.