Article | 12. 2014 Vol. 32, Issue. 6
Inhibition of in Vitro Growth of Three Soil-borne Turfgrass Diseases by Antagonistic Bacteria from Composted Liquid Manure



Department of Horticultural Science & Biotechnology, Seoul National University1
Korea Turfgrass Research Institute2
Research Institute of Agriculture and Life Sciences, Seoul National University3




2014.12. 879:886


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This study was conducted to test in vitro the antagonistic effect of composted liquid manure (CLM) against soil-borne turfgrass pathogenic fungi, Rhizoctonia solani AG-2-2 (IIIB) (brown patch), R. solani AG-2-2 (IV) (large patch), and Sclerotinia homoeocarpa (dollar spot) for environmentally friendly turfgrass management. CLMs were collected from 9 livestock excretion treatment facilities around the country including Gunwi (GW), Hapcheon (HC), Hoengseong (HS), Icheon (IC), Iksan (IS), Muan (MA), Nonsan (NS), and Yeoju (YJ). CLMs of IC, GW, and IS showed significant (p < 0.05) mycelium growth inhibition that was 17.8%, 20.4%, and 48.0% against R. solani AG-2-2 (IIIB), R. solani AG-2-2 (IV), and S. homoeocarpa, respectively. A total of 110 bacterial isolates were obtained from the CLMs that showed antagonistic effects. Among them, 5, 4, and 10 microbe isolates showed promising antifungal activity against mycelium growth of R. solani AG-2-2 (IIIB), R. solani AG-2-2 (IV), and S. homoeocarpa, respectively. The bacterial isolates ICIIIB60, GWIV70, and ISSH20 effectively inhibited the mycelial growth of three soil-borne turfgrass pathogens. Selected bacterial isolates were identified as Alcaligenes sp., Bacillus licheniformis Ab2, and B. subtilis C7-3 through 16s rDNA gene sequence analysis. Among 5 fungicides, the most compatible fungicide with ICIIIB60, GWIV70, and ISSH20 was tebuconazol, toclofos-methyl and toclofos-methyl, respectively. These findings suggested that CLMs could be effectively used not only as organic liquid fertilizer sources but also as biological control agents for soil-borne turfgrass diseases such as brown patch, large patch, and dollar spot.



1. Boulter, J.I., G.J. Boland, and J.T. Trevors. 2002. Evaluation of composts for suppression of dollar spot (Sclerotinia homoeocarpa) of turfgrass. Plant Dis. 86:405-410.  

2. Cheng, Z., P.S. Grewal, B.R. Stinner, K.A. Hurto, and H.B. Hamza. 2008 Effects of long-term turfgrass management practices on soil nematode community and nutrient pools. Appl. Soil Ecol. 38:174-184.  

3. El-Masry, M., A. Khalil, M. Hassouna, and H. Ibrahim. 2002. In situ and in vitro suppressive effect of agricultural composts and their water extracts on some phytopathogenic fungi. World J. Microbiol. Biotechnol. 18:551-558.  

4. Garling D.C. and M.J. Boehm. 2001. Temporal effects of compost and fertilizer applications on nitrogen fertility of golf course turfgrass. Agron. J. 93:548-555.  

5. Ham, S.K., Y.S. Kim, and C.H. Park. 2010. The growth effects of creeping bentgrass by SCB (slurry composting and biofilteration) liquid fertilizer application. Kor. Turfgrass Sci. 24:56-61.  

6. Ham, S.K., Y.S. Kim, T.S. Kim, K.S. Kim, and C.H. Park. 2009. The effect of SCB (slurry composting and biofilteration) liquid fertilizer on growth of creeping bentgrass. Kor. Turfgrass Sci. 23:91-100.  

7. He, Y.C., J.H. Xu, J.H. Su, and L. Zhou. 2010. Bioproduction of glycolic acid from glycolonitrile with a new bacterial isolate of Alcaligenes sp. ECU0401. Appl. Biochem. Biotechnol. 160:1428-1440.  

8. Jeon, S.J., S.R. Kim, K.S. Rho, D.Y. Choi, D.K. Kim, and M.G. Lee. 2012. Physicochemical characteristics of liquid fertilizer made from pig manure in Korea. J. Livestock Housing Environ. 18:221-228.  

9. Jung, W.C., T.S. Shin, K.S. Do, W.K. Kim, J.H. Lee, and K.H. Choi. 2006. Development of antagonistic microorganism for biological control of blight of turfgrass. Res. Plant Dis. 12:260-266.  

10. Kang, B.K., H.H. Jung, and K.S. Kim. 2010. Effect of slurry composted and biofiltered solution and an organic fertilizer on the growth of zoysiagrass. Hort. Environ. Biotechnol. 51:507-512.  

11. Kim, H.Y., K.S. Gwak, H.Y. Kim, K.O. Ryu, P.G. Kim, D.H. Cho, J.Y. Choi, and I.G. Choi. 2011. Effect of treatment amounts of slurry composting and biofilteration liquid fertilizer on growth characteristics and bioethanol production of yellow poplar. Kor. Soc. Wood Sci. Tech. 39:459-468.  

12. Kim, Y.S., S.K. Ham, and H.J. Lim. 2012. Monitoring of soil chemical properties and pond water quality in golf courses after application of SCB liquid fertilizer. Asian J. Turfgrass Sci. 26:44-53.  

13. Lee, S.J., K.K. Shim, Y.K. Kim, and K.Y. Hue. 1998. Suppression of Rhizoctonia spp. by antagonistic microorganisms and their compatibility with fungicides. Kor. Turfgrass Sci. 12:23-30.  

14. Lim, H.J., Y.S. Kim, and S.K. Ham. 2011. Screening of cellulose decomposing microorganisms for functional improvement for SCB (slurry composting and biofiltering) liquid fertilizer. Asian J. Turfgrass Sci. 25:48-51.  

15. Lim, T.J., S.D. Hong, S.H. Kim, and J.M. Park. 2008. Evaluation of yield and quality from red pepper for application rates of pig slurry composting biofilteration. Kor. J. Environ. Agr. 27:171-177.  

16. Liu, S. and R. Baker. 1980. Mechanism of biological control in soils suppressive to Rhizoctonia solani. Phytopathology 70:404-412.  

17. Moody, A.R. and D. Gindrat. 1977. Biological control of cucumber black root rot by Gliocladium roseum. Phytopathology 67: 1159-1162.  

18. Naidu, Y., S. Meon, and Y. Siddiqui. 2012. In vitro and in vivo evaluation of microbial-enriched compost tea on the development of powdery mildew on melon. BioControl 57:827-836.  

19. Nakasaki, K., S. Hiraoka, and H. Nagata. 1998. A new operation for producing disease-suppressive compost from grass clippings. Appl. Environ. Microbiol. 64:4015-4020.  

20. Nelson, E.B. and C. Craft. 1991. Introduction and establishment of strains of Enterobacter cloacae in golf course turf for the biological control of dollar spot. Plant Dis. 75:510-514.  

21. Nelson, E.B. and M.J. Boehm. 2002. Compost-induced suppression of turf grass diseases. BioCycle 43:51-51.  

22. Oh, H.M., J.H. Kang, C.H. Lee, C.S. Park, S.K. Ahn, B.D. Yoon, and Y.H. Kho. 1994. Isolation and characterization of a naphthalene-degrading strain, Alcaligenes sp. A111. Kor. J. Appl. Microbiol. Biotechnol. 22:423-429.  

23. Park, J.M., T.J. Lim, S.E. Lee, and I.B. Lee. 2011. Effect of pig slurry fertigation on soil chemical properties and growth and development of cucumber (Cucumis sativus L.). Kor. J. Soil Sci. Fert. 44:194-199.  

24. Park, S.J., S.Y. Lee, J.H. Ryu, H.H. Jung and K.S. Kim. 2012. Optimum application amount, timing, and frequency of slurry composted and biofiltered liquid fertilizer for Zoysia japonica ‘Millock’. Kor. J. Hort. Sci. Technol. 30:635-641.  

25. Ruppel, E.G., R. Baker, G.E. Harman, J.P. Hubbard, R.J. Hecker, and I. Chet. 1983. Field tests of Trichoderma harzianum Rifai aggr. as a biocontrol agent of seedling disease in several crops and Rhizoctonia root rot of sugar beet. Crop Prot. 2:399-408.  

26. Seo, Y.H., B.O. Cho, H.K. Choi, A.S. Kang, B.C. Jeong, and Y.S. Jung. 2010. Impact of continuous application of swine slurry on changes in soil properties and yields of tomatoes and cucumbers in a greenhouse. Kor. J. Soil Sci. Fert. 43: 446-452.  

27. Shim, G.Y. and H.K. Kim. 2000. Control of large patch caused by Rhizoctonia solani AG2-2 by combined application of antagonists and chemicals in golf courses. Kor. Turfgrass Sci. 13:131-138.  

28. Tu, C.M. 1980. Influence of five pyrethroid insecticides on microbial populations and activities in soil. Microb. Ecol. 5:321-327.