Article | 08. 2014 Vol. 32, Issue. 4
Assessment of Substantial Equivalence and Environmental Risk for Event Selection of Genetically Modified Chrysanthemum

Department of Bioresources Engineering, Sejong University1
Synnong Floriculture Nursery Company2
Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration3
Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, Rural Development Administration4

2014.08. 517:524


As an assessment of substantial equivalence and environmental risks of branchless chrysanthemum GM lines (LeLS69, LeLS76, LeLS80) developed in Korea, we investigated their degree of branchlessness and developmental characteristics including growth and flowering, as well as the stability of populations of insect pests and soil microbes. The GM lines were compared with a control variety, ‘Jinba’. LeLS80 showed the highest level of branchlessness among the three GM lines, whereas abnormal axillary buds developed in lines LeLS76 and LeLS69. There were no significant differences between the growth characteristics of ‘Jinba’ and the GM lines. The branchlessness LeLS80 was increased during the summer season, suggesting that it is a useful germplasm for developing elite branchless cultivars. These GM lines showed no significant differences in the occurrence of five insect pests including aphids, mites, leaf miners, thrips, and whiteflies. Similarly, for soil microbes including bacteria and actinomycetes, there were no significant differences between ‘Jinba’ and the GM lines. However, there was a higher occurrence of fungi in the GM lines compared to ‘Jinba’. Our findings suggest that ‘Jinba’ and the GM lines are substantially equivalent, although further monitoring studies are needed to confirm this.

1. Al-Ahmad, H. and J. Gressel. 2005. Assessing and managing biological risks of plants used for bioremediation, including risks of transgene flow. Z Naturforsch C 60:154-165.  

2. Baek, H.J., S.I. Sohn, M.R. Cho, G.S. Lee, Y.J. Oh, J.S. Park, KJ. Lee, S.D. Oh, S.C. Suh, and T.H. Ryu. 2010. Development of protocol for analyzing pollinator insect-mediated gene transfer from GM crop. Korean J. Intl. Agri. 22:293-297.  

3. Cresswell, J.E. 1999. The influence of nectar and pollen availability on pollen transfer by individual flowers of oilseed rape (Brassica napus) when pollinated by bumblebees (Bombus lapidraius). J. Ecol. 87:670-677.   

4. Deguchi, H. 2002. Method of vanishing lateral bud in chrysanthemum ‘Iwanohakusen’. Kyushu Agric. Res. 64:189. (Abstr.)  

5. De Vries, J. and W. Wackernagel. 2004. Microbial horizontal gene transfer and the DNA release from transgenic crop plants. Plant Soil. 266:91-104.  

6. Faust, J.E. and R.D. Heins. 1992. High night temperatures do not cause poor lateral branching of chrysanthemum. HortScience 27:981-982.  

7. Gotoh, S. and T. Tomimitsu. 1999. Effect of growth condition control by closing greenhouse and GA treatment on chrysanthemum cultivar ‘Iwanohakusen’. Kyushu Agric. Res. 61:193. (Abstr.)  

8. Huh, E.J., B.H. Han, S.K. Park, S.Y. Lee, M.J. Kil, and C.H. Park. 2013. Inhibition of chrysanthemum axillary buds via transformation with the antisense tomato lateral suppressor gene is season dependent. Hort. Environ. Biotechnol. 54:280-281.  

9. Huh, E.J., Y.R. Lee, S.Y. Choi, K.R. Do, and C.H. Park. 2007. Genotypic differences of axillary budding in response to tem-perature and ethephon treatment in non-branching chrysanthemums. Hort. Environ. Biotechnol. 48:370-375.  

10. Huh, M. 2012. A study on the assessment of the effects of genetically modified plants on soil microorganisms. PhD Diss., Inha Univ., Incheon, Korea.  

11. Kaneko, E. and T. Morita. 2002. Effect of growing temperature on formation of axillary buds in non-branching type chrysanthemum. J. Japan. Soc. Hort. Sci 71(Suppl.):171. (Abstr.)  

12. Lippert, L.F., B.O. Bergh, and P.G. Smith. 1965. Gene list for the pepper. J. Heredity 56:30-34.  

13. Liu, B., Q. Zeng, F. Yan, H. Xu, C. Xu. 2005. Effects of transgenic plants on soil microorganisms. Plant Soil 271:1-13.  

14. Malayer, J.C. and A.T. Guard. 1964. A comparative developmental study of the mutant sideshootless and normal tomato plants. Amer. J. Bot. 51:140-143.  

15. Mapelli, S. and L. Lombardi. 1982. A comparative auxin and cytokinin study in normal and to-2 mutant tomato plants. Plant Cell Physiol. 23:751-757.  

16. Nap, J.P., J. Bijvoet, and W.J. Stiekema. 1992. Biosafety of kanamycin-resistant transgenic plants. Transgenic Res. 1:239-249.  

17. National Academy of Agricultural Science (NAAS). 2005. Guideline for environmental risk assessment of genetically modified crops: A general consideration. NAAS, Suwon, Korea.  

18. Pierre, J. 2001. The role of honeybees (Apis mellifera) and other insect pollinators in gene flow between oilseed rape (Brassica napus) and wild radish (Raphanus raphanistrum). Acta Hortic. 561:47-51.  

19. Rural Development Administration (RDA). 2010. The LMO safety supervision statute book. RDA, Suwon, Korea.  

20. Shin, H.K. 1995. Study on mechanism and regulation of the expression of branchlessness in chrysanthemum. PhD Diss., Kyungpook National Univ., Daegu, Korea.  

21. Shin, H.K., J.K. Choi, and S.T. Choi. 1996. Effect of planting time and pinching on aspects of axillary bud abortion in branchless chrysanthemums. J. Kor. Soc. Hort. Sci. 37:442-446.  

22. Shin, H.K., J.Y. Ko, and S.T., Choi. 1998. Pattern of branchless expression and selection of new branchless line in chrysanthemum. Kor. J. Hort. Sci. Technol. 16:78. (Abstr.)  

23. Sohn, S.I., B.O. Ahn, H.Y. Chi, B.K. Cho, M.S. Cho, and K.S. Shin. 2012. Assessment of microbial community in paddy soils cultivated with Bt and Nakdong rice. Korean J. Soil. Sci. Fert. 45:829-835.  

24. Sohn, S.I., Y.J. Oh, S.D. Oh, M.K. Kim, T.H. Ryu, K.J. Lee, S.C. Suh, H.J. Baek, and J.S. Park. 2010. Molecular analysis of microbial community in soils cultivating Bt Chinese cabbage. Korean J. Environ. Agric. 29:293-299.  

25. Stewart, C.N., M.D. Halfhill, and S.I. Warwick. 2003. Transgenic introgression from genetically modified crops to their wild relatives. Nat. Rev. Gen. 4:806-817.  

26. Tsuhiya, T. 2001. Techniques for growth and flowering in August on chrysanthemum ‘Iwanohakusen’. Bull. Fukui Agr. Exp. Sta. 38:23-29.  

27. Yi, H.B., M.C. Kwon, J.E. Park, C.G. Kim, K.W. Park, B.K. Lee, and H.M. Kim. 2007. Effect of the anthracnose resistant transgenic red pepper on the arthropod communities in a confined field. Kor. J. Environ. Biol. 25:326-335.   

28. Yoo, Y.K., K.H. Cha, K.B. Ahn, I.T. Hwang, K.C. Cho, and H.G. Ahn. 2011. The disease and insect pest occurrence in protected cultivation of standard chrysanthemum during the last three years in Jeonnam. Kor. J. Hort. Sci. Technol. 29(Suppl. I):169. (Abstr.)