Vegetable Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration1
The antimicrobial and antioxidant activities of different cultivars and parts of broccoli were investigated. The screening of antimicrobial activities for the floret and leaf extracts of 11 cultivars against selected gram positive (Staphylococcus aureus and Listeria monocytogenes) and gram negative bacteria (Escherichia coli O157:H7 and Salmonella enteritidis) were conducted. The AMG cultivar showed the highest antimicrobial activity against S. aureus with the floret and leaf extracts (12.83 and 13.00 mm). Antibacterial activity against L. monocytogenes was strongest with floret extract of NJ cultivar (13.58 mm) and leaf extract of YDR cultivar (13.92 mm). Moreover, the size of inhibition zone against L. monocytogenes was bigger than those of 4 kinds of pathogenic bacteria. Both floret and leaf extracts of Grd cultivar showed the highest antimicrobial activity against E. coli O157:H7, but there was no difference between floret and leaf extracts. Floret extract of NJ cultivar and leaf extract of NY and 0c cultivars were effective against S. enteritidis whereas leaf extract exhibited better antimicrobial effect than floret extract. These results showed that floret extract of NJ and leaf extract of 0c had the highest antioxidant activity which was 39.90 and 43.64%, respectively. The antioxidant activity of leaf extract was 1.5 times higher than that of floret extract. All cultivars except NJ showed that electron donating ability of leaf extract was higher than that of floret extract.
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