Article | 06. 2014 Vol. 32, Issue. 3
Major Metabolites Involved in Skin Blackening of ‘Niitaka’ Pear Stored under Cold Temperature



Department of Horticultural Science and Biotechnology, Seoul National University1
Research Institute of Agriculture and Life Sciences, Seoul National University2




2014.06. 359:365


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Oriental pear (Pyrus communis L. cv ‘Niitaka’) was stored at 0°C for 5 months and major metabolites involved in blackening of the peel were analyzed by untargeted GC-MS and targeted HPLC methods. In this study, peels of sound and skin-blackened pears were analyzed and compared. Skin-blackened fruit was clearly characterized by a distinctive pattern in changes which included a decrease of malic acid, succinic acid, and ascorbic acid, while an increase of fumaric acid, threonine, and gluconic acid, which indicated both reduced metabolic activity and anti-oxidative capacity of the cells. Chlorogenic acid was a major phenolic compound and the peel of sound fruit showed high levels of free phenolic compounds compared than the peel of skin-blackened fruit which are believed to be related to oxidation of phenolics in skin-blackened tissue. The changes or profiling of major metabolites by targeted or untargeted analysis method could become a useful tool for understanding physiology, disorder mechanism, and identifying metabolic networks connecting primary and secondary metabolism in postharvest research.



1. Choi, S.J., Y.P. Hong, and Y.B. Kim. 1995. Prestorage treatments to prevent fruit skin blackening during cold storage of Japanese pear ‘Shingo’ (‘Niitaka’). J. Kor. Sci. Hort. Sci. 36:218-223.  

2. Coseteng, M.Y. and C.Y. Lee. 1987. Changes in apple poly-phenoloxidase and polyphenol concentrations in relation to degree of browning. J. Food Sci. 52:985-989.  

3. Dai, J. and R.J. Mumper. 2010. Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules 15:7312-7352.  

4. Franck, C., J. Lammertyn, Q.T. Ho, P. Verboven, B. Verlinden, and B.M. Nicolaï. 2007. Browning disorders in pear fruit. Postharvest Biol. Technol. 43:1-13.  

5. Franck, C., M. Baetens, J. Lammertyn, N. Scheerlinck, M.W. Davey, and B.M. Nicolaï. 2003. Ascorbic acid mapping to study core breakdown development in ‘Conference’ pears. Postharvest Biol. Technol. 30:133-142.  

6. Furuta, M., S. Asano, and S. Imai. 1992. Prevention of browning of the peel of pears by polyethylene film packaging. Packaging Technol. Sci. 5:91-100.  

7. Hernández-Sánchez, N., B.P. Hills, P. Barreiro, and N. Marigheto. 2007. An NMR study on internal browning in pears. Postharvest Biol. Technol. 44:260-270.  

8. Hong, Y.P., S.K. Lee, Y.M. Park, and H.S. Park. 2008. Develop-mental anatomy and features of the exocarp as related with fruit skin disorders in ‘Niitaka’ pear fruit. J. Japan. Soc. Hort. Sci. 77:382-387.   

9. Kang, H.K. and S.K. Lee. 2003. Effects of prestorage treatment by EDTA-Ca and heating on skin blackening and quality during cold storage in ‘Niitaka’ pear fruits. J. Kor. Soc. Hort. Sci. 44:52-56.   

10. Kang, H.K., Y.K. Yoo, and S.K. Lee. 2003. Effects of prestorage heat treatment on changes of phenolic compound contents and incidence of skin blackening in ‘Niitaka’ pear fruits during cold storage. J. Kor. Soc. Hort. Sci. 44:197-200.  

11. Kim, S.H., S.O. Yang, K.H. Kim, Y.S. Kim, K.H. Liu, Y.R. Yoon, D.H. Lee, C.H. Lee, G.S. Hwang, M.W. Chung, K.H. Choi, and H.K. Choi. 2009. Research trends, applications, and domestic research promotion stratigies of metabolomics. Kor. Soc. Biotechnol. Bioenginner. J. 24:113-121.   

12. Larrigaudiere, C., I. Lentheric, and M. Vendrell. 1998. Relationship between enzymatic browning and internal disorders in controlled- atmosphere stored pears. J. Sci. Food Agric. 78:232-236.  

13. Lau, O.L. 1998. Effect of growing season, harvest maturity, waxing, low O and elevated CO on flesh browning disorders in ‘Braeburn’ apples. Postharvest Biol. Technol. 14:131-141.  

14. Ma, S.S. and P.M. Chen. 2003. Storage disorder and ripening behavior of ‘Doyenne du Comice’ pears in relation to storage conditions. Postharvest Biol. Technol. 28:281-294.  

15. Oms-Oliu, G., M.L.A.T.M. Hertog, B. Van de Poela, J. Ampofo- Asiama, A.H. Geeraerd, and B.M. Nicolaï. 2011. Metabolic characterization of tomato fruit during preharvest development, ripening, and postharvest shelf-life. Postharvest Biol. Technol. 62:7-16.  

16. Park, Y.S. 1999a. Carbon dioxide-induced flesh browning develop-ment as related to phenolic metabolism in ‘Niitaka’ pear during storage. J. Kor. Soc. Hort. Sci. 40:567-570.  

17. Park, Y.S. 1999b. Changes in the incidence of fruit skin blackening, phenolic acids, and ethanol production of non-astrigent ‘Fuyu’ persimmon fruits during CA and MAP storage. J. Kor. Soc. Hort. Sci. 40:83-87.  

18. Pedreschi, R., C. Franck, J. Lammertyn, A. Erban, J. Kopka, M. Hertong, B. Verlinden, and B. Nicolaï. 2009. Metabolic profiling of ‘Conference’ pears under low oxygen stress. Postharvest Biol. Technol. 51:123-130.  

19. Pesis, E. 2005. The role of the anaerobic metabolites, acetaldehyde and ethanol, in fruit ripening, enhancement of fruit quality and fruit deterioration. Postharvest Biol. Technol. 37:1-19.  

20. Rudell, D.R. and J.R. Mattheis. 2009. Superficial scald development and related metabolism in modified by postharvest light irradiation. Postharvest Biol. Technol. 51:174-182.  

21. Seo, J.H., Y.S. Hwang, J.P. Chun, and J.C. Lee. 2001. Changes of phenolic compounds and occurrence of skin browning, and characterization of partially purified polyphenol oxidases in oriental pear fruits. J. Kor. Soc. Hort. Sci. 42:184-188.   

22. Sweetlove, L.J., J.L. Heazlewood, V. Herald, R. Holtzapffel, D.A. Day, C.J. Leaver, and A.H. Miller. 2002. The impact of oxidative stress in Arabidopsis mitochondria. Plant J. 32:891-904.  

23. Trinchero, G.D., G.O. Sozzi, F. Covatta, and A.A. Fraschina. 2004. Inhibition of ethylene action by 1-methylcyclopropene extends postharvest life of “Bartlett” pears. Postharvest Biol. Technol. 32:193-204.  

24. Veltman, R.H., I. Lenthéric, L.H.W. Van der Plas, and H.W. Peppelenbos. 2003. Internal browning in pear fruit (Pyrus communis L. cv Conference) may be a result of a limited availability of energy and antioxidants. Postharvest Biol. Technol. 28:295-302.  

25. Yang, Y.J. 1997. Inhibition of the skin blackening by postharvest factors in ‘Niitaka’ pear fruit. J. Kor. Soc. Hort. Sci. 38:730-733.