Article | 4. 2014 Vol. 32, Issue. 2
Ethylene Production and Expression of Two Ethylene Biosynthetic Genes in Senescing Flowers of Hosta ventricosa

College of Chemistry and Life Sciences, Zhejiang Normal University1

2014.4. 261:268


Senescence of Hosta ventricosa flowers was firstly characterized as ethylene-sensitive since the deterioration of the tepal was accompanied by increased endogenous ethylene biosynthesis. The full-length cDNAs and DNAs of 1-aminocyclopropane-1- carboxylic acid (ACC) synthase (ACS) and ACC oxidase (ACO) involved in ethylene biosynthesis were cloned from H. ventricosa flowers. The HvACS ORF with 1347 bp and two introns, encoded a polypeptide of 448 amino acids showing 79% homology with that in Musa acuminata. The HvACO ORF contained 957 bp and three introns, encoding a 318-residue polypeptide showing 83% homology with that in Narcissus tazetta. The timing of the induction of HvACS expression was in correspond to the timing of the increase in ethylene production, and that the up-regulation of HvACO transcript was closely correlated with an elevated ethylene production, but underwent a down-regulation in wounded leaves with elevated ethylene emission. The results, together with expression analysis in vegetative tissues, suggested that both HvACS and HvACO were specifically regulated by flower senescence.

1. Cao, G., L. Xue, Y. Li, and K. Pan. 2011. The relative importance of architecture and resource competition in allocation to pollen and ovule number within inflorescences of Hosta ventricosa varies with the resource pools. Ann. Bot. 107:1413-419.  

2. Hunter, D.A. and M.S. Reid. 2001. A simple and rapid method for isolating high quality RNA from flower petals. Acta Hort. 543:147-152.  

3. Jones, M.L. 2003. Ethylene biosynthetic genes are differentially regulated by ethylene and ACC in carnation styles. Plant Growth Regul. 40:129-138.  

4. Jones, M.L. and W.R. Woodson. 1999. Differential expression of three members of the 1-aminocyclopropane-1-carboxylate synthase gene family in carnation. Plant Physiol. 119:755-764.  

5. Lasserre, E., T. Bouquin, J.A. Hernandez, J. Bull, J.C. Pech, and C. Balagué. 1996. Structure and expression of three genes encoding ACC oxidase homologs from melon (Cucumis melo L.). Mol. Gen. Genet. 251:81-90.  

6. Lin, Z., S. Zhong, and D. Grierson. 2009. Recent advances in ethylene research. J. Exp. Bot. 60:3311-3336.  

7. Llop-Tous, I., C.S. Barry, and D. Grierson. 2000. Regulation of ethylene biosynthesis in response to pollination in tomato flowers. Plant Physiol. 123:971-978.  

8. Müller, R., S. Lind-Iversen, B.M. Stummann, and M. Serek. 2000. Expression of genes for ethylene biosynthetic enzymes and an ethylene receptor in senescing flowers of miniature potted roses. J. Hort. Sci. Biotech. 75:12-18.  

9. Rao, G., Y. Wang., D. Zhang, D. Liu, F. Li, and H. Lu. 2012. Isolation and characterisation of an HpSHP gene from Hosta plantaginea. Mol. Biol. Rep. 39:6887-6894.  

10. Rogers, H.J. 2006. Programmed cell death in floral organs: How and why do flowers die? Ann. Bot. 97:309-315.  

11. Savin, K.L., S.C. Baudioette, M.W. Graham, M.Z. Michael, G.D. Nugent, C.Y. Lu, S.F. Chandler, and E.C. Cornish. 1995. Antisense ACC oxidase RNA delays carnation petal senescence. HortScience 30:970-972.  

12. Shadrack, K.G. and M. Shadrack. 2010. The book of little Hostas: 200 small, very small, and mini varieties. Timber Press, USA.  

13. ten Have, A. and E.J. Woltering. 1997. Ethylene biosynthetic genes are differentially expressed during carnation (Dianthus caryophyllus L.) flower senescence. Plant Mol. Biol. 34:89-97.  

14. Trusov, Y. and J.R. Botella. 2006. Silencing of the ACC synthase gene ACACS2 causes delayed flowering in pineapple [Ananas comosus (L.) Merr.]. J. Exp. Bot. 57:3953-3960.  

15. Wagstaff, C., U. Chanasut, F.J. Harren, L.J. Laarhoven, B. Thomas, H.J. Rogers, and A.D. Stead. 2005. Ethylene and flower longevity in Alstroemeria: Relationship between tepal senescence, abscission and ethylene biosynthesis. J. Exp. Bot. 56:1007-1016.  

16. Wang, X., Y. Zhang, J. Zhang, C. Cheng, and X. Guo. 2007. Molecular characterization of a transient expression gene encoding for 1-aminocyclopropane-1-carboxylate synthase in cotton (Gossypium hirsutum L.). J. Biochem. Mol. Biol. 40:791-800.  

17. Weterings, K., M. Pezzotti, M. Cornelissen, and C. Mariani. 2002. Dynamic 1-aminocyclopropane-1-carboxylate-synthase and -oxidase transcript accumulation patterns during pollen tube growth in tobacco styles. Plant Physiol. 130:1190-1200.  

18. Woodson, W.R., K.Y. Park, A. Drory, P.B. Larsen, and H. Wang. 1992. Expression of ethylene biosynthetic pathway transcripts in senescing carnation flowers. Plant Physiol. 99:526-532.  

19. Xue, J., Y. Li, H. Tan, F. Yang, N. Ma, and J. Gao. 2008. Expression of ethylene biosynthetic and receptor genes in rose floral tissues during ethylene-enhanced flower opening. J. Exp. Bot. 59:2161- 2169.  

20. Zhang, Z., J.S. Ren, I.J. Clifton, and C.J. Schofield. 2004. Crystal structure and mechanistic implications of 1-aminocyclopropane- 1-carboxylic acid oxidase-the ethylene-forming enzyme. Chem. Biol. 11:1383-1394.