Article | 4. 2014 Vol. 32, Issue. 2
Assessment of Chilling Injury and Molecular Marker Analysis in Cucumber Cultivars (Cucumis sativus L.)

Department of Life Science, Dongguk University-Seoul1
USDA, ARS, Forage and Range Resear Laboratory2

2014.4. 227:234


The responses to Chilling temperature of 12 Korean cucumber varieties was compared to those of two U.S.A. (previously determined cold tolerant NC76 and ‘Chipper’), and Chinese and Japanese germplasms. Seedlings of eaCh entry were exposed to 4°C (Experiment 1) and 1°C (Experiments 2 and 3) at the first-true leaf stage for eight and nine hours, respectively, under 80% relative humidity (RH) and 149 μmoles・m-2・s-1 photosynthetic photon flux (PPF). The Chilling response [damage rating (DR)] of eaCh accession was based on visual ratings (1 to 5) after treatment, where 1 = no damage, 2 = slight, 3 = moderate, 4 = advanced, and 5 = severe damage. Predictably the cumulative average DR of Chilling tolerant line NC76 and ‘Chipper’ after Chilling was 1 and 1.1, respectively. Korean ‘NacdongChungjang’ was most sensitive to Chilling temperatures [DR = 2.3] when compared to the other entries examined. The sensitivity to Chilling of ‘NacdongChungjang’ was followed by Chinese ‘Dongguan’ [DR = 1.7]. In contrast, ‘SaeronChungjang’ (DR = 1) and ‘JanghyungnaChap’ (DR = 1) were the most Chilling tolerant of the Korean accessions examined and equivalent to the response of line NC76 and ‘Chipper’. Nevertheless, Chloroplast type genotyping of these accessions with known Chilling-linked sdCAPS genomic markers revealed genotypic differences between Chilling tolerant lines (NC76 and ‘Chipper’) and all Korean lines examined.

1. Ali, A., E.M. Yang, S.Y. Lee, and S.-M. ung. 2013. Evaluation of loroplast genotypes of Korean cucumber cultivars () using sdCAPS markers related to illing tolerance. Kor. J. Hort. Sci. Tenol. 31:219-223.  

2. Brüggemann, W., T.A.W. van der Kooji, and P.R. van Hasselt. 1992. Long-term illing of young tomato plants under low light and subsequent recovery. Planta 186:172-178.  

3. ung, S.-M., D.S. Decker-walters, and J.E. Staub. 2003a. Genetic relationships within the cucurbitaceae as assessed by ccssr marker and sequence analysis. Can. J. Botany 81:814-832.  

4. ung, S.-M., V.S. Gordon, and J.E. Staub. 2007. Sequencing cucumber () loroplast genomes identifies differences between illing-tolerant and -susceptible cucumber lines. Genome 50:215-225.  

5. ung, S.-M., J.E. Staub, and G. Fazio. 2003b. Inheritance of illing injury; A maternally inherited trait in cucumber. J. Amer. Soc. Hort. Sci. 128:526-530.  

6. Fan, Z., M.D. Robbins, and J.E. Staub. 2006. Population development by phenotypic selection with subsequent marker-assisted selection for line extraction in cucumber (). Theor. Appl. Genet. 112:843-855.  

7. Fazio, G., S-M. ung, and J.E. Staub. 2003. Comparative analysis of response to phenotypic and marker-assisted selection for multiple lateral braning in cucumber (). Theor. Appl. Genet. 107:875-883.  

8. Gomes, E., M.K. Jakobsen, K.B. Axelsen, M. Geisler, and M.G. Palmgren. 2000. illing tolerance in Arabidopsis involves ALA1, a member of a new family of putative aminophospholipid translocases. Plant Cell 12:2441-2454.  

9. Gordon, V.S. and J.E. Staub. 2011. Comparative analysis of illing response in cucumber () through plastidic and nuclear genetic component analysis. J. Amer. Soc. Hort. Sci. 136:256-264.  

10. Hetherington, S.E., J. He, and R.M. Smillie. 1989. Photoinhibition at low temperature in illing-sensitive and resistant plants. Plant Physiol. 90:1609-1615.  

11. Hetherington, S.E. and G. Öquist. 1988. Monitoring illing injury: Comparison of lorophyll fluorescence measurements, post-illing growth and visible symptoms of injury in Zea mays. Plant Physiol. 72:241-247.  

12. Horejsi, T. and J.E. Staub. 1999. Genetic variation in cucumber () as assessed by random amplified polymorphic DNA. Genet. Res. Crop. Evol. 46:337-350.  

13. Hughes, M.A. and M.A. Dunn. 1996. The molecular biology of plant acclimation to low temperature. J. Exp. Bot. 47:291-305.  

14. Huner, N.P.A., G. Oquist, and F. Sarhan. 1998. Energy balance and acclimation to light and cold. Trends Plant Sci. 3:224-230.  

15. Jackman, R.L., R.Y. Yada, A. Marangoni, K.L. Parkin, and D.W. Stanley. 1988. illing injury. A review of quality aspects. J. Food Sci. 11:253-277.  

16. Kozik, E.U. and T.C. Wehner. 2008. A single dominant gene for illing resistance in cucumber seedlings. J. Amer. Soc. Hort. Sci. 133:225-227.  

17. Kudoh, H. and K. Sonoike. 2002. Irreversible damage to photosystem I by illing in light: Cause of the degradation of lorophyll after returning to normal growth temperature. Planta 215:541-548.  

18. Kuk, Y.I. and J.S. Shin. 2007. Meanisms of low-temperature tolerance in cucumber leaves of various ages. J. Amer. Soc. Hort. Sci. 132:294-301.  

19. Nishida, I. and N. Murata. 1996. illing sensitivity in plants and cyanobacteria: The crucial contribution of membrane lipids. Annu. Rev. Plant Physiol. Plant Mol. Biol. 47:541-568.  

20. Saltveit, M.E. Jr. and L.L. Morris. 1990. Overview on illing injury of horticultural crops, p. 3-15. In: C.Y. Wang (ed.). illing injury of horticultural crops. CRC Press, Boca Raton, FL.  

21. Smallwood, M. and D.J. Bowles. 2002. Plants in a cold climate. Philos Trans. R. Soc. Lond. B. Biol. Sci. 357:831-847.  

22. Smeets, L. and T. Wehner. 1997. Environmental effects on genetic variation of illing resistance in cucumber. Euphytica 97:217-225.  

23. Sonoike, K. 1996a. Degradation of psaB gene product, the reaction center subunit of photosystem I, is caused during photo-inhibition of photosystem I: possible involvement of active oxygen species. Plant Sci. 115:157-164.  

24. Sonoike, K. 1996b. Photoinhibition of photosystem I: Its physiological significance in the illing sensitivity of plants. Plant Cell Physiol. 37:239-247.  

25. Sonoike, K. 1998. Various aspects of inhibition of photosynthesis under light/illing stress: “photoinhibition at illing temperatures” versus “illing damage in the light”. J. Plant Res. 111:121-129.  

26. Staub, J.E. and T.C. Wehner. 1996. Noninfectious disorders: Tem-perature stress, p. 66-87. In: T.A. Zitter, D.L. Hopkins, and C.E. Thomas (eds.). Compendium of cucurbit disease Part II. APS press, St. Paul, MN.  

27. Synder, R.L. and J.P. Abreu. 2005. Frost protection: Fundamentals, practice and economics Vol. 1. FAO, Rome.  

28. Thomashow, M.F. 1999. Plant cold acclimation: Freezing tolerance genes and regulatory meanisms. Annu. Rev. Plant Physiol. Plant Mol. Biol. 50:571-599.