Greenbio Research Center, Korea Research Institute of Bioscience and Biotechnology1
Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University2
School of Life Sciences and Bioengineering, The Nelson Mandela African Institute of Science and Technology3
Research Institute for Subtropical Agriculture and Biotechnology, Jeju National University4
Microbiological Resource Center, Korea Research Institute of Bioscience and Biotechnology5
We established a high throughput screening system of African yam tuber lines which contain high contents of total carotenoids, flavonoids, and phenolic compounds using ultraviolet-visible (UV-VIS) spectroscopy and Fourier transform infrared (FT-IR) spectroscopy in combination with multivariate analysis. The total carotenoids contents from 62 African yam tubers varied from 0.01 to 0.91 μg･g-1 dry weight (wt). The total flavonoids and phenolic compounds also varied from 12.9 to 229 μg･g-1 and from 0.29 to 5.2 mg･g-1 dry wt. FT-IR spectra confirmed typical spectral differences between the frequency regions of 1,700-1,500, 1,500-1,300 and 1,100-950 cm-1, respectively. These spectral regions were reflecting the quantitative and qualitative variations of amide I, II from amino acids and proteins (1,700-1,500 cm-1), phosphodiester groups from nucleic acid and phospholipid (1,500-1,300 cm-1) and carbohydrate compounds (1,100-950 cm-1). Principal component analysis (PCA) and subsequent partial least square- discriminant analysis (PLS-DA) were able to discriminate the 62 African yam tuber lines into three separate clusters corresponding to their taxonomic relationship. The quantitative prediction modeling of total carotenoids, flavonoids, and phenolic compounds from African yam tuber lines were established using partial least square regression algorithm from FT-IR spectra. The regression coefficients (R2) between predicted values and estimated values of total carotenoids, flavonoids and phenolic compounds were 0.83, 0.86, and 0.72, respectively. These results showed that quantitative predictions of total carotenoids, flavonoids, and phenolic compounds were possible from FT-IR spectra of African yam tuber lines with higher accuracy. Therefore we suggested that quantitative prediction system established in this study could be applied as a rapid selection tool for high yielding African yam lines.
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