Highland Agriculture Research Center, National Institute of Crop Science, Rural Development Administration1
Department of Bio-Health Technology, Kangwon National University2
Department of Plant Science, Gangneung-Wonju National University3
This study was conducted to determine the optimal MS medium strength to improve sprouting rate of apical meristem of strawberry ‘Seolhyang’ in vitro. Strawberry apical meristems at size (0.2 mm to 0.3 mm) with leaf primordials were cultured on the MS media with four strength levels (1/4×, 1/3×, 1/2×, and 1×) and the sprouting rate and growth characteristics were evaluated after eight weeks after cultivation. Shoot rate of ‘Daewang’ apical meristems was 93.6% whereas ‘Seolhyang’ apical meristems were sprouted with 31.6% on 1× MS medium strength. Different sprouting rates were observed in ‘Seolhyang’ apical meristem with 31.6% in 1× medium, 75.0% in 1/2× medium, and 94.4% in 1/3× medium. The sprouting rate was improved with the decrease of medium strength, but the shoot rate in 1/4× medium decreased up to 54.5%. Shoot length was 0.9 cm in 1× medium, 1.2 cm in 1/2× medium, 1.6 cm in 1/3× medium, and 1.9 cm in 1/4× medium. Shoot length was longer as medium strength decreased and numbers of leaves and roots were not significant differences among the medium strengths. As a result, sprouting rate was highest and plant growth was best in 1/3× MS medium compared to the others.
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