Article | . 2014 Vol. 32, Issue. 1
Investigation of Colony Forming Unit (CFU) of Microorganisms in the Paprika-grown Greenhouses Using Open and Closed Soilless Culture Systems

Department of Plant Science, Seoul National University1
Research Institute for Agriculture and Life Sciences, Seoul National University2

2014.. 46:52


This study was conducted to compare colony forming unit (CFU) of microorganisms in closed and open soilless culture systems for estimating the possibility for potential disease occurrence. Samples were collected at four different positions in four commercial greenhouses with closed or open soilless culture system using rock wool or coir as substrate, respectively. The distance between sampling positions was 3 cm starting from the substrate and the surface area of each position was 25 cm2. The CFU of fungi was significantly higher in the open system, while that of bacteria was not significantly different but showed relatively lower in the closed system. Samples collected at the plastic surface of the substrates where little environmental effects occurred from drainage showed lower CFU than any other positions. The principal component analysis showed that samples collected on the drainage pathway highly affected the changes in microbial population in the greenhouse. These results indicated that greenhouses with closed soilless culture are expected to have more advantageous conditions for restraining the microbial growth, resulting in the lower potential of disease occurence in greenhouse ecosystem.

1. Agrios, G.N. 2005. Plant pathology. 5th ed. Elsevier Academic Press, Burlington, MA, USA.  

2. Ahn, T.I, J.H. Shin, and J.E. Son. 2010. Analysis of changes in ion concentration with time and drainageratio under EC-based nutrient control in closed-loop soilless culture for sweet pepper plants (Capsicum annumL. ‘Boogie’). J. Bio-env. Control 19: 298-304.  

3. Chang, E.H., R.S. Chung, and Y.H. Tsai. 2007. Effect of different application rates of organic fertilizer on soil enzyme activity and microbial population. Soil Sci. Plant Nutr. 53:132-140.  

4. Domeno, I., N. Irigoyenand, and J. Muro. 2009. Evolution of organic matter and drainages in wood fibre and coconut fibre substrates. Sci. Hort. 122:269-274.  

5. Etchells, J.L., T.A. Bell, R.N. Costilow, C.E. Hoodand, and T.E. Anderson. 1973. Influence of temperature and humidity on microbial, enzymatic, and physical changes of stored, pickling cucumbers. Appl. Microbiol. 26:943-950.  

6. Flory, S.L. and K. Clay. 2013. Pathogen accumulation and long- term dynamics of plant invasions. J. Ecol. 101:607-613.  

7. Lee, J.G., B.Y. Leeand, and H.J. Lee. 2006. Accumulation of phytotoxic organic acids in reused nutrient solution during hydroponic cultivation of lettuce (Lactuca sativa L.). Sci. Hort. 110:119-128.  

8. Ko, M.T, T.I. Ahn. Y.Y. Cho, and J.E. Son. 2013. Uptake of nutrients and water by paprika (Capsicum annuum L.) as affected by renewal period of recycled nutrient solution in closed soilless culture. Hort. Environ. Biotechnol. 54:412-421.  

9. Mcavoy, R.J. 1994. Nitrate-nitrogen movement through the soil- profile beneath a containerized greenhouse crop irrigated with 2 leaching fractions and 2 wetting agent levels. J. Amer. Soc. Hort. Sci. 119:446-451.  

10. Rowe, R.C. and J.D. Farley. 1978. Control of fusarium crown and root-rot of greenhouse tomatoes by inhibiting recolonization of steam-disinfested soil with a captafol drench. Phytopathology 68:1221-1224.  

11. Schon, M.K. and M.P. Compton. 1997. Comparison of cucumbers grown in rockwool or perlite at two leaching fractions. HortTechnology 7:30-33.  

12. Sridhar, K.R. and F. Barlocher. 2000. Initial colonization, nutrient supply, and fungal activity on leaves decaying in streams. Appl. Environ. Microbiol. 66:1114-1119.  

13. van Maanen, A. and X.M. Xu. 2003. Modelling plant disease epidemics. Eur. J. Plant Pathol. 109:669-682.  

14. Van Os, E.A. 1991. Closed business systems for less pollution from greenhouses. Acta. Hort. 294:49-58  

15. Van Os, E.A. 1999. Closed soilless growing systems: A sustainable solution for Dutch greenhouse horticulture. Water Sci. Technol. 39:105-112.  

16. Zekki, H., L. Gauthier, and A. Gosselin. 1996. Growth, productivity, and mineral composition of hydroponically cultivated greenhouse tomatoes, with or without nutrient solution recycling. J. Amer. Soc. Hort. Sci. 121:1082-1088.